biostudies-literatureUnknown4(12)Miyamoto TThe ability to quench reactive oxygen species (ROS) overproduced in plant chloroplasts under light stress conditions is essential for securing plant photosynthetic performance and agricultural yield. Although genetic engineering can enhance plant stress resistance, its widespread application faces limitations due to challenges in successful transformation across plant species and public acceptance concerns. This study proposes a nontransgenic chemical approach using a designed chimeric peptide that scavenges ROS within plant chloroplasts for managing light stress. The chimeric peptide was strategically designed by combining cell-penetrating and chloroplast-targeting sequences, each with antioxidant ability against destructive ROS such as hydroxyl radical (•OH) and singlet oxygen (<sup>1</sJACS Au4691-4699https://www.ebi.ac.uk/biostudies/studies/S-EPMC11672152biostudies-literatureA Chimeric Peptide for Shielding Plant Photosynthetic Systems against Excess Light Stress via Chloroplast-Targeted ROS Quenching.PMC11672152Numata KMiyamoto TMorey-Yagi SRfalseA Chimeric Peptide for Shielding Plant Photosynthetic Systems against Excess Light Stress via Chloroplast-Targeted ROS Quenching.The ability to quench reactive oxygen species (ROS) overproduced in plant chloroplasts under light stress conditions is essential for securing plant photosynthetic performance and agricultural yield. Although genetic engineering can enhance plant stress resistance, its widespread application faces limitations due to challenges in successful transformation across plant species and public acceptance concerns. This study proposes a nontransgenic chemical approach using a designed chimeric peptide that scavenges ROS within plant chloroplasts for managing light stress. The chimeric peptide was strategically designed by combining cell-penetrating and chloroplast-targeting sequences, each with antioxidant ability against destructive ROS such as hydroxyl radical (•OH) and singlet oxygen (<sup>1</s2024-01-01T00:00:00Z2024 Dec2025-04-04T02:36:07.465Z2025-04-04T02:36:07.465ZS-EPMC116721523973591710.1021/jacsau.4c00478