biostudies-literatureEstevan-Morio ESpanish Ministry of Science and Innovation (MICINN)Consejo Superior de Investigaciones CientíficasGovernment of CataloniaMinisterio de Ciencia, Innovación y UniversidadesJAE-Intro fellowshipConsolidated Research Group (SGR) project of the Generalitat de Catalunya1604https://www.ebi.ac.uk/biostudies/studies/S-EPMC11674741biostudies-literatureUnknown14(12)Cytophaga is a genus of Gram-negative bacteria occurring in soil and the gut microbiome. It is closely related to pathogenic <i>Flavobacterium</i> spp. that cause severe diseases in fish. Cytophaga strain L43-1 secretes cytophagalysin (CPL1), a 137 kDa peptidase with reported collagenolytic and gelatinolytic activity. We performed highly-confident structure prediction calculations for CPL1, which identified 11 segments and domains, including a signal peptide for secretion, a prosegment (PS) for latency, a metallopeptidase (MP)-like catalytic domain (CD), and eight immunoglobulin (Ig)-like domains (D3-D10). In addition, two short linkers were found at the D8-D9 and D9-D10 junctions, and the structure would be crosslinked by four disulfide bonds. The CPL1 CD was found closest to ulilysin froBiomoleculesDissecting Cytophagalysin: Structural and Biochemical Studies of a Bacterial Pappalysin-Family Metallopeptidase.PMC11674741RYC2020-029773-IPRE2020-0967312021SGR00423JAEINT_22_02654PID2021-128682OA-I00Bushi EEstevan-Morio ERamirez-Larrota JSEckhard UfalseDissecting Cytophagalysin: Structural and Biochemical Studies of a Bacterial Pappalysin-Family Metallopeptidase.Cytophaga is a genus of Gram-negative bacteria occurring in soil and the gut microbiome. It is closely related to pathogenic <i>Flavobacterium</i> spp. that cause severe diseases in fish. Cytophaga strain L43-1 secretes cytophagalysin (CPL1), a 137 kDa peptidase with reported collagenolytic and gelatinolytic activity. We performed highly-confident structure prediction calculations for CPL1, which identified 11 segments and domains, including a signal peptide for secretion, a prosegment (PS) for latency, a metallopeptidase (MP)-like catalytic domain (CD), and eight immunoglobulin (Ig)-like domains (D3-D10). In addition, two short linkers were found at the D8-D9 and D9-D10 junctions, and the structure would be crosslinked by four disulfide bonds. The CPL1 CD was found closest to ulilysin fro2024-01-01T00:00:00Z2024 Dec2026-05-26T20:10:17.763Z2026-05-25T03:07:09.427ZS-EPMC116747413976631210.3390/biom14121604