biostudies-literatureMitsueda RReiko MitsuedaNaohiko SekiHiroko Toda60https://www.ebi.ac.uk/biostudies/studies/S-EPMC11677261biostudies-literatureUnknown10(6)Our recently created RNA-sequence-based microRNA (miRNA) expression signature in breast cancer clinical specimens revealed that some <i>miR-30</i> family members were significantly downregulated in cancer tissues. Based on TCGA database analyses, we observed that among the <i>miR-30</i> family members, <i>miR-30a-3p</i> (the passenger strand derived from pre-<i>miR-30a</i>) was significantly downregulated in breast cancer (BC) clinical specimens, and its low expression predicted worse prognoses. Ectopic expression assays showed that <i>miR-30a-3p</i> transfected cancer cells (MDA-MB-157 and MDA-MB-231) had their aggressive phenotypes significantly suppressed, e.g., their proliferation, migration, and invasion abilities. These data indicated that <i>miR-30a-3p</i> acted as a tumor-suppressiNon-coding RNAIdentification of Tumor-Suppressive <i>miR-30a-3p</i> Controlled Genes: <i>ANLN</i> as a Therapeutic Target in Breast Cancer.PMC1167726124K1174622K0870524K12641Toda HShinden YNagata AYasudome RKato MMitsueda RNakajo ASeki NTomioka YfalseIdentification of Tumor-Suppressive <i>miR-30a-3p</i> Controlled Genes: <i>ANLN</i> as a Therapeutic Target in Breast Cancer.Our recently created RNA-sequence-based microRNA (miRNA) expression signature in breast cancer clinical specimens revealed that some <i>miR-30</i> family members were significantly downregulated in cancer tissues. Based on TCGA database analyses, we observed that among the <i>miR-30</i> family members, <i>miR-30a-3p</i> (the passenger strand derived from pre-<i>miR-30a</i>) was significantly downregulated in breast cancer (BC) clinical specimens, and its low expression predicted worse prognoses. Ectopic expression assays showed that <i>miR-30a-3p</i> transfected cancer cells (MDA-MB-157 and MDA-MB-231) had their aggressive phenotypes significantly suppressed, e.g., their proliferation, migration, and invasion abilities. These data indicated that <i>miR-30a-3p</i> acted as a tumor-suppressi2024-01-01T00:00:00Z2024 Nov2026-04-19T03:27:46.726Z2025-04-19T21:26:55.634ZS-EPMC116772613972860510.3390/ncrna10060060