biostudies-literatureWatanuki SJapan Society for the Promotion of Science (JSPS)a Grant-in-Aid for JSPS Fellow from the Japan Society for the Promotion of Science (JSPS)Japan Racing Association (JRA)a grant from the Livestock Promotional Subsidycollaborative research fundcollaborative research fund from Nippon Gene Co., Ltd.1111https://www.ebi.ac.uk/biostudies/studies/S-EPMC11677995biostudies-literatureUnknown13(12)The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the "Liquid Dual-CoCoMo assay", that uses the coordination of common motif (CoCoMo) degenerate primers. This method can detect two genes simultaneously using a FAM-labeled minor groove binder (MGB) probe for the BLV long terminal repeat (LTR) region and a VIC-labeled MGB probe for the <i>BoLA-DRA</i> gene. In this study, we evaluated the diagnostic and analytical performance of the Dual-CoCoMo assay targeting the LTR region by comparing its performance against the commercially available Takara multiplex assay targeting the <iPathogens (Basel, Switzerland)BLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting <i>pol</i> Region.PMC11677995from the Japan Racing Association (JRA)23KJ0412from Nippon Gene Co., Ltd.Okami MIzawa MSaitou EMatsumoto YBao AWatanuki SShoji KAida YfalseBLV-CoCoMo Dual qPCR Assay Targeting LTR Region for Quantifying Bovine Leukemia Virus: Comparison with Multiplex Real-Time qPCR Assay Targeting <i>pol</i> Region.The proviral load (PVL) of the bovine leukemia virus (BLV) is a useful index for estimating disease progression and transmission risk. Real-time quantitative PCR techniques are widely used for PVL quantification. We previously developed a dual-target detection method, the "Liquid Dual-CoCoMo assay", that uses the coordination of common motif (CoCoMo) degenerate primers. This method can detect two genes simultaneously using a FAM-labeled minor groove binder (MGB) probe for the BLV long terminal repeat (LTR) region and a VIC-labeled MGB probe for the <i>BoLA-DRA</i> gene. In this study, we evaluated the diagnostic and analytical performance of the Dual-CoCoMo assay targeting the LTR region by comparing its performance against the commercially available Takara multiplex assay targeting the <i2024-01-01T00:00:00Z2024 Dec2026-04-19T03:26:12.941Z2026-04-19T03:13:58.753ZS-EPMC116779953977037010.3390/pathogens13121111