{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Du L"],"funding":["Shaanxi Key R&D Program；DAYU Bioengineering (Xi'an) Industrial Development Research Institute"],"pagination":["1360"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11680129"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(12)"],"pubmed_abstract":["<b>Background/Objectives:</b><i>Clostridium perfringens</i> is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. <b>Methods:</b> In this study, the <i>C. perfringens</i> α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created. The corresponding DNA vaccine cassettes driven by a CMV promoter were constructed and were cloned into a yeast shuttle vector. Recombinant yeast strains transformed with these shuttle vectors were then prepared as the YCMs for the subsequent oral immunization of m"],"journal":["Vaccines"],"pubmed_title":["Oral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against <i>Clostridium perfringens</i> Induce Effective Intestinal Immunity and Modulate Gut Microbiota."],"pmcid":["PMC11680129"],"funding_grant_id":["2024NC-YBXM-098 and 2021NY-027；DYSW2021-023"],"pubmed_authors":["Zhang W","Yang X","Liu Y","Xu K","Cai C","Zhu Y","Jia S","Wang C","Ma X","Wei Z","Du L"],"additional_accession":[]},"is_claimable":false,"name":"Oral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against <i>Clostridium perfringens</i> Induce Effective Intestinal Immunity and Modulate Gut Microbiota.","description":"<b>Background/Objectives:</b><i>Clostridium perfringens</i> is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. <b>Methods:</b> In this study, the <i>C. perfringens</i> α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created. The corresponding DNA vaccine cassettes driven by a CMV promoter were constructed and were cloned into a yeast shuttle vector. Recombinant yeast strains transformed with these shuttle vectors were then prepared as the YCMs for the subsequent oral immunization of m","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-04-18T03:27:03.938Z","creation":"2026-04-18T03:13:29.244Z"},"accession":"S-EPMC11680129","cross_references":{"pubmed":["39772022"],"doi":["10.3390/vaccines12121360"]}}