biostudies-literatureDu LShaanxi Key R&D Program；DAYU Bioengineering (Xi'an) Industrial Development Research Institute1360https://www.ebi.ac.uk/biostudies/studies/S-EPMC11680129biostudies-literatureUnknown12(12)<b>Background/Objectives:</b><i>Clostridium perfringens</i> is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. <b>Methods:</b> In this study, the <i>C. perfringens</i> α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created. The corresponding DNA vaccine cassettes driven by a CMV promoter were constructed and were cloned into a yeast shuttle vector. Recombinant yeast strains transformed with these shuttle vectors were then prepared as the YCMs for the subsequent oral immunization of mVaccinesOral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against <i>Clostridium perfringens</i> Induce Effective Intestinal Immunity and Modulate Gut Microbiota.PMC116801292024NC-YBXM-098 and 2021NY-027；DYSW2021-023Zhang WYang XLiu YXu KCai CZhu YJia SWang CMa XWei ZDu LfalseOral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against <i>Clostridium perfringens</i> Induce Effective Intestinal Immunity and Modulate Gut Microbiota.<b>Background/Objectives:</b><i>Clostridium perfringens</i> is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. <b>Methods:</b> In this study, the <i>C. perfringens</i> α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created. The corresponding DNA vaccine cassettes driven by a CMV promoter were constructed and were cloned into a yeast shuttle vector. Recombinant yeast strains transformed with these shuttle vectors were then prepared as the YCMs for the subsequent oral immunization of m2024-01-01T00:00:00Z2024 Dec2026-04-18T03:27:03.938Z2026-04-18T03:13:29.244ZS-EPMC116801293977202210.3390/vaccines12121360