biostudies-literatureUnknown14(1)Kros JMIn previous work we discovered that T lymphocytes play a prominent role in the rise of brain metastases of ER-negative breast cancers. In the present study we explored how T lymphocytes promote breast cancer cell penetration through the blood brain barrier (BBB). An in vitro BBB model was employed to study the effects of T lymphocytes on BBB trespassing capacity of three different breast carcinoma cell lines. Differential protein expression was explored by comparing the proteomes of the breast cancer cells before and after co-culture with activated T lymphocytes using liquid chromatography-mass spectrometry (LC-MS). siRNA was used to silence protein expression in the breast cancer cells to study contribution to in vitro BBB passage. Furthermore, protein expression in primary breast cancer Scientific reports31516https://www.ebi.ac.uk/biostudies/studies/S-EPMC11682172biostudies-literatureT cell induced expression of Coronin-1A facilitates blood-brain barrier transmigration of breast cancer cells.PMC11682172Mustafa DAMKros JMDik WABelcaid ZLuider TMZeneyedpour LPedrosa RMSMfalseT cell induced expression of Coronin-1A facilitates blood-brain barrier transmigration of breast cancer cells.In previous work we discovered that T lymphocytes play a prominent role in the rise of brain metastases of ER-negative breast cancers. In the present study we explored how T lymphocytes promote breast cancer cell penetration through the blood brain barrier (BBB). An in vitro BBB model was employed to study the effects of T lymphocytes on BBB trespassing capacity of three different breast carcinoma cell lines. Differential protein expression was explored by comparing the proteomes of the breast cancer cells before and after co-culture with activated T lymphocytes using liquid chromatography-mass spectrometry (LC-MS). siRNA was used to silence protein expression in the breast cancer cells to study contribution to in vitro BBB passage. Furthermore, protein expression in primary breast cancer 2024-01-01T00:00:00Z2024 Dec2026-05-26T14:02:54.489Z2025-04-04T21:48:49.365ZS-EPMC116821723973310710.1038/s41598-024-83301-x