{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kjar A"],"funding":["NICHD NIH HHS","NCATS NIH HHS","NCRR NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NIA NIH HHS","National Institute on Aging","NEI NIH HHS","NIDDK NIH HHS","The Chan Zuckerberg Initiative","NINDS NIH HHS","NCI NIH HHS","NIGMS NIH HHS","National Science Foundation"],"pagination":["114874"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11682736"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(11)"],"pubmed_abstract":["Human neural organoid models have become an important tool for studying neurobiology. However, improving the representativeness of neural cell populations in such organoids remains a major effort. In this work, we compared Matrigel, a commercially available matrix, to a neural cadherin (N-cadherin) peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. We determined that peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad hydrogels mapped more closely to human fetal populations and produced neurons with more spontaneous excitatory postsynaptic currents relative to Matrigel. These results provide compelling evidence that matrix-tethered signaling peptides can influence neural organoid differentiation, opening an avenue to control stem cell fate. Moreover, outcomes from this work showcase the technical utility of GelMA-Cad as a simple and defined hydrogel alternative to Matrigel for neural organoid culture."],"journal":["Cell reports"],"pubmed_title":["Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids."],"pmcid":["PMC11682736"],"funding_grant_id":["UL1 TR002243","S10 RR025677","T32 AG058524","P30 DK058404","P30 EY008126","P50 HD103537","R01 DK103831","F99 NS125829","UL1 RR024975","P30 CA068485","G20 RR030956","R01 NS110665","R35 GM128915"],"pubmed_authors":["Fernandez M","Drake LE","Curry CW","Chavarria D","Kim H","Moen RK","Haschert MR","Abdulrahman AM","Grueter B","Kjar A","Robertson G","Lau KS","Gama V","Yates A","Zepeda JC","O'Grady BJ","Marguerite NT","Lippmann ES","Simmons AJ","Brunger JM"],"additional_accession":[]},"is_claimable":false,"name":"Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids.","description":"Human neural organoid models have become an important tool for studying neurobiology. However, improving the representativeness of neural cell populations in such organoids remains a major effort. In this work, we compared Matrigel, a commercially available matrix, to a neural cadherin (N-cadherin) peptide-functionalized gelatin methacryloyl hydrogel (termed GelMA-Cad) for culturing cortical neural organoids. We determined that peptide presentation can tune cell fate and diversity in gelatin-based matrices during differentiation. Of particular note, cortical organoids cultured in GelMA-Cad hydrogels mapped more closely to human fetal populations and produced neurons with more spontaneous excitatory postsynaptic currents relative to Matrigel. These results provide compelling evidence that matrix-tethered signaling peptides can influence neural organoid differentiation, opening an avenue to control stem cell fate. Moreover, outcomes from this work showcase the technical utility of GelMA-Cad as a simple and defined hydrogel alternative to Matrigel for neural organoid culture.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-01T23:03:29.633Z","creation":"2025-04-04T21:48:51.536Z"},"accession":"S-EPMC11682736","cross_references":{"pubmed":["39423129"],"doi":["10.1016/j.celrep.2024.114874"]}}