{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(4)"],"submitter":["Lin Y"],"pubmed_abstract":["Notch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.<sup>1</sup>."],"journal":["STAR protocols"],"pagination":["103519"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11683232"],"repository":["biostudies-literature"],"pubmed_title":["Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system."],"pmcid":["PMC11683232"],"pubmed_authors":["Li W","Luo L","Che W","Xu C","Lin Y","Jiang D","Zhu G","Singh S","Wang Z","Feng C"],"additional_accession":[]},"is_claimable":false,"name":"Protocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.","description":"Notch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.<sup>1</sup>.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T08:12:03.753Z","creation":"2025-04-04T08:12:03.753Z"},"accession":"S-EPMC11683232","cross_references":{"pubmed":["39661510"],"doi":["10.1016/j.xpro.2024.103519"]}}