biostudies-literatureUnknown5(4)Lin YNotch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.¹.STAR protocols103519https://www.ebi.ac.uk/biostudies/studies/S-EPMC11683232biostudies-literatureProtocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.PMC11683232Li WLuo LChe WXu CLin YJiang DZhu GSingh SWang ZFeng CfalseProtocol for tyramide signal amplification immunohistochemical detection of Notch1 signaling in the vascular system.Notch signaling is a pivotal regulator in the vascular system that is essential for development, angiogenesis, and maintaining vascular homeostasis. Here, we present a protocol for tyramide signal amplification (TSA) immunohistochemistry, tailored explicitly for detecting Notch signaling components in vascular tissues. We describe steps for utilizing tailored antigen retrieval techniques, specific blocking solutions, and a complex of avidin/biotin-horseradish peroxidase conjugate with tyramide, along with optimized washing steps. For complete details on the use and execution of this protocol, please refer to Zhu et al.¹.2024-01-01T00:00:00Z2024 Dec2025-04-04T08:12:03.753Z2025-04-04T08:12:03.753ZS-EPMC116832323966151010.1016/j.xpro.2024.103519