biostudies-literatureJeon BCNational Research Foundation of Korea (NRF)Korea Research Institute of Bioscience and Biotechnology (KRIBB)National Research Council of Science and Technology (National Research Council of Science & Technology)68-82https://www.ebi.ac.uk/biostudies/studies/S-EPMC11686140biostudies-literatureUnknown22(1)V(D)J recombination secures the production of functional immunoglobulin (Ig) genes and antibody diversity during the early stages of B-cell development through long-distance interactions mediated by cis-regulatory elements and trans-acting factors. O-GlcNAcylation is a dynamic and reversible posttranslational modification of nuclear and cytoplasmic proteins that regulates various protein functions, including DNA-binding affinity and protein-protein interactions. However, the effects of O-GlcNAcylation on proteins involved in V(D)J recombination remain largely unknown. To elucidate this relationship, we downregulated O-GlcNAcylation in a mouse model by administering an O-GlcNAc inhibitor or restricting the consumption of a regular diet. Interestingly, the inhibition of O-GlcNAcylation in miCellular & molecular immunologyDynamic O-GlcNAcylation governs long-range chromatin interactions in V(D)J recombination during early B-cell development.PMC11686140KGM9942421NTC26423112021R1I1A20579452021R1A2C10124772022M3E5F1016693ZYM93823122020R1I1A3073845Park AKJeon BCPark YKim TDKim YJNa KMLee JHwang HLim JSong MRAn DPark SKfalseDynamic O-GlcNAcylation governs long-range chromatin interactions in V(D)J recombination during early B-cell development.V(D)J recombination secures the production of functional immunoglobulin (Ig) genes and antibody diversity during the early stages of B-cell development through long-distance interactions mediated by cis-regulatory elements and trans-acting factors. O-GlcNAcylation is a dynamic and reversible posttranslational modification of nuclear and cytoplasmic proteins that regulates various protein functions, including DNA-binding affinity and protein-protein interactions. However, the effects of O-GlcNAcylation on proteins involved in V(D)J recombination remain largely unknown. To elucidate this relationship, we downregulated O-GlcNAcylation in a mouse model by administering an O-GlcNAc inhibitor or restricting the consumption of a regular diet. Interestingly, the inhibition of O-GlcNAcylation in mi2025-01-01T00:00:00Z2025 Jan2026-06-06T19:57:32.708Z2026-06-04T03:12:32.755ZS-EPMC116861403962760910.1038/s41423-024-01236-9