{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["240(1)"],"submitter":["Wang N"],"pubmed_abstract":["Megalin is a multiple-ligand receptor that contributes to protein reabsorption in the kidney. Recently, megalin was found to act as a novel endocytic receptor for prorenin. Internalization depended on the (pro)renin receptor. This receptor is an accessory protein of vacuolar H<sup>+</sup>-ATPase (V-ATPase), a complex consisting of 14 subunits and two accessory proteins. Here we explored whether V-ATPase elements other than the (P)RR affect megalin-mediated prorenin uptake. Using RNAi technology, we inhibited each individual V-ATPase subunit in megalin-expressing BN16 cells. Subsequently, we quantified megalin expression and the uptake of prorenin. To unravel the underlying molecular mechanisms, we investigated the adaptor proteins autosomal recessive hypercholesterolemia (ARH) and Disabled"],"journal":["Journal of cellular physiology"],"pagination":["e31518"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11694337"],"repository":["biostudies-literature"],"pubmed_title":["Vacuolar H&lt;sup&gt;+&lt;/sup&gt;-ATPase and Megalin-Mediated Prorenin Uptake: Focus on Elements Beyond the (Pro)Renin Receptor."],"pmcid":["PMC11694337"],"pubmed_authors":["Wang N","Lu X","Jan Danser AH"],"additional_accession":[]},"is_claimable":false,"name":"Vacuolar H&lt;sup&gt;+&lt;/sup&gt;-ATPase and Megalin-Mediated Prorenin Uptake: Focus on Elements Beyond the (Pro)Renin Receptor.","description":"Megalin is a multiple-ligand receptor that contributes to protein reabsorption in the kidney. Recently, megalin was found to act as a novel endocytic receptor for prorenin. Internalization depended on the (pro)renin receptor. This receptor is an accessory protein of vacuolar H<sup>+</sup>-ATPase (V-ATPase), a complex consisting of 14 subunits and two accessory proteins. Here we explored whether V-ATPase elements other than the (P)RR affect megalin-mediated prorenin uptake. Using RNAi technology, we inhibited each individual V-ATPase subunit in megalin-expressing BN16 cells. Subsequently, we quantified megalin expression and the uptake of prorenin. To unravel the underlying molecular mechanisms, we investigated the adaptor proteins autosomal recessive hypercholesterolemia (ARH) and Disabled","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T12:02:52.249Z","creation":"2025-04-04T12:02:52.249Z"},"accession":"S-EPMC11694337","cross_references":{"pubmed":["39745029"],"doi":["10.1002/jcp.31518"]}}