biostudies-literatureLiang JTianjin Municipal Science and Technology ProgramNatural Science Foundation of Beijing MunicipalityNational Natural Science Foundation of China2022 Tianjin Postgraduate Scientific Research Innovation Project589-605https://www.ebi.ac.uk/biostudies/studies/S-EPMC11695388biostudies-literatureUnknown480(1)Non-SMC (Structural Maintenance of Chromosomes) condensin I complex subunit H (NCAPH) has been shown to facilitate progression and predict adverse prognostic outcome in many cancer types. However, the function of NCAPH in gliomas is still unclear. Series of experiments were taken to uncover the function of NCAPH in glioma. The expression of NCAPH and potential mechanism regulating progression of glioma was verified by bioinformatics analysis. Lentiviral transfection was used for establishment of loss-of-function and gain-of-function cell lines. CCK-8 assay and Colony-formation assay were used to evaluate proliferation. Transwell assay and Cell wound healing assay were used to assess migration and invasion. Cell cycle and apoptosis were measured by flow cytometry. Protein and RNA were quantMolecular and cellular biochemistryNCAPH serves as a prognostic factor and promotes the tumor progression in glioma through PI3K/AKT signaling pathway.PMC11695388No.7232228No. 21JCYBJC00800No.2022SKY222No.82102753No. 81872063Yang XJin WLi YFan JYu SLi TLiang JYun DWang XZhang CfalseNCAPH serves as a prognostic factor and promotes the tumor progression in glioma through PI3K/AKT signaling pathway.Non-SMC (Structural Maintenance of Chromosomes) condensin I complex subunit H (NCAPH) has been shown to facilitate progression and predict adverse prognostic outcome in many cancer types. However, the function of NCAPH in gliomas is still unclear. Series of experiments were taken to uncover the function of NCAPH in glioma. The expression of NCAPH and potential mechanism regulating progression of glioma was verified by bioinformatics analysis. Lentiviral transfection was used for establishment of loss-of-function and gain-of-function cell lines. CCK-8 assay and Colony-formation assay were used to evaluate proliferation. Transwell assay and Cell wound healing assay were used to assess migration and invasion. Cell cycle and apoptosis were measured by flow cytometry. Protein and RNA were quant2025-01-01T00:00:00Z2025 Jan2025-04-04T13:41:44.804Z2025-04-04T13:41:44.804ZS-EPMC116953883858778610.1007/s11010-024-04976-4