biostudies-literatureJones RMNational Health Research InstitutesNINDS NIH HHSNCI NIH HHSNIH HHS30240https://www.ebi.ac.uk/biostudies/studies/S-EPMC11697013biostudies-literatureUnknown14(1)A long-standing goal of neuroimaging is the non-invasive volumetric assessment of whole brain function and structure at high spatial and temporal resolutions. Functional ultrasound (fUS) and ultrasound localization microscopy (ULM) are rapidly emerging techniques that promise to bring advanced brain imaging and therapy to the clinic with the safety and low-cost advantages associated with ultrasound. fUS has been used to study cerebral hemodynamics at high temporal resolutions while ULM has been used to study cerebral microvascular structure at high spatial resolutions. These two methods have complementary spatio-temporal characteristics, making them ideally suited for multimodal imaging, but both suffer from limitations associated with transcranial ultrasound imaging. Here, these two methoScientific reportsNon-invasive 4D transcranial functional ultrasound and ultrasound localization microscopy for multimodal imaging of neurovascular response.PMC11697013RF1 NS113285R01 CA220681R01-CA220681RF1-NS113285Belgharbi HLee HRSantibanez FJones RMMunot SDayton PADeRuiter RMPinton GFFavorov OVfalseNon-invasive 4D transcranial functional ultrasound and ultrasound localization microscopy for multimodal imaging of neurovascular response.A long-standing goal of neuroimaging is the non-invasive volumetric assessment of whole brain function and structure at high spatial and temporal resolutions. Functional ultrasound (fUS) and ultrasound localization microscopy (ULM) are rapidly emerging techniques that promise to bring advanced brain imaging and therapy to the clinic with the safety and low-cost advantages associated with ultrasound. fUS has been used to study cerebral hemodynamics at high temporal resolutions while ULM has been used to study cerebral microvascular structure at high spatial resolutions. These two methods have complementary spatio-temporal characteristics, making them ideally suited for multimodal imaging, but both suffer from limitations associated with transcranial ultrasound imaging. Here, these two metho2024-01-01T00:00:00Z2024 Dec2025-04-04T12:02:23.425Z2025-04-04T12:02:23.425ZS-EPMC116970133974714310.1038/s41598-024-81243-y