biostudies-literatureOdio CDU.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)NIAID NIH HHS191https://www.ebi.ac.uk/biostudies/studies/S-EPMC11697199biostudies-literatureUnknown16(1)The standard dengue virus (DENV) neutralization assay inconsistently predicts dengue protection. We compare how IgG ELISA, envelope domain III (EDIII), or non-structural protein 1 (NS1) binding antibodies, and titers from plaque reduction neutralization tests (PRNTs) using standard and mature viruses are associated with dengue. The ELISA measures IgG antibodies that bind to inactivated DENV1-4. The EDIII and NS1 assays measure binding antibodies, and the PRNTs measure neutralizing antibodies to each specific DENV serotype. Healthy children (n = 1206) in Cebu, Philippines were followed for 5 years. ELISA IgG≥3 was associated with reduced dengue probability relative to naïve children (3% vs. 10%, p = 0.007). Serotype-specific antibodies binding EDIII or NS1 had no association with dengue risNature communicationsDengue virus IgG and neutralizing antibody titers measured with standard and mature viruses are protective.PMC11697199P01AI106695P01 AI106695Katzelnick LCAdams CAogo RAFirdous SYlade Mde Silva AMOdio CDAgrupis KAVoirin CJDahora Hein LAbad Fernandez MMpingabo PIWhite LJCrisostomo MVCoello Escoto ARaimundi Rodriguez GDeen JDaag JVfalseDengue virus IgG and neutralizing antibody titers measured with standard and mature viruses are protective.The standard dengue virus (DENV) neutralization assay inconsistently predicts dengue protection. We compare how IgG ELISA, envelope domain III (EDIII), or non-structural protein 1 (NS1) binding antibodies, and titers from plaque reduction neutralization tests (PRNTs) using standard and mature viruses are associated with dengue. The ELISA measures IgG antibodies that bind to inactivated DENV1-4. The EDIII and NS1 assays measure binding antibodies, and the PRNTs measure neutralizing antibodies to each specific DENV serotype. Healthy children (n = 1206) in Cebu, Philippines were followed for 5 years. ELISA IgG≥3 was associated with reduced dengue probability relative to naïve children (3% vs. 10%, p = 0.007). Serotype-specific antibodies binding EDIII or NS1 had no association with dengue ris2025-01-01T00:00:00Z2025 Jan2026-06-02T02:04:52.551Z2025-04-05T22:50:51.93ZS-EPMC116971993974784610.1038/s41467-024-53916-9