{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McIntyre ABR"],"funding":["EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 Marie Skłodowska-Curie Actions (H2020 Excellent Science - Marie Skłodowska-Curie Actions)","Swiss National Science Foundation","European Molecular Biology Organization (EMBO)"],"pagination":["390"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11700124"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Nuclear speckles are membraneless organelles that associate with active transcription sites and participate in post-transcriptional mRNA processing. During the cell cycle, nuclear speckles dissolve following phosphorylation of their protein components. Here, we identify the PP1 family as the phosphatases that counteract kinase-mediated dissolution. PP1 overexpression increases speckle cohesion and leads to retention of mRNA within speckles and the nucleus. Using APEX2 proximity labeling combined with RNA-sequencing, we characterize the recruitment of specific RNAs. We find that many transcripts are preferentially enriched within nuclear speckles compared to the nucleoplasm, particularly chromatin- and nucleus-associated transcripts. While total polyadenylated RNA retention increases with n"],"journal":["Nature communications"],"pubmed_title":["Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention."],"pmcid":["PMC11700124"],"funding_grant_id":["192622","ALTF 978-2019","101029269","310030"],"pubmed_authors":["Walser S","Rai AK","Tschan AB","Meyer K","Fujita K","McIntyre ABR","Pelkmans L"],"additional_accession":[]},"is_claimable":false,"name":"Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.","description":"Nuclear speckles are membraneless organelles that associate with active transcription sites and participate in post-transcriptional mRNA processing. During the cell cycle, nuclear speckles dissolve following phosphorylation of their protein components. Here, we identify the PP1 family as the phosphatases that counteract kinase-mediated dissolution. PP1 overexpression increases speckle cohesion and leads to retention of mRNA within speckles and the nucleus. Using APEX2 proximity labeling combined with RNA-sequencing, we characterize the recruitment of specific RNAs. We find that many transcripts are preferentially enriched within nuclear speckles compared to the nucleoplasm, particularly chromatin- and nucleus-associated transcripts. While total polyadenylated RNA retention increases with n","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-04-08T19:53:20.037Z","creation":"2026-04-08T14:31:26.829Z"},"accession":"S-EPMC11700124","cross_references":{"pubmed":["39755675"],"doi":["10.1038/s41467-024-55469-3"]}}