biostudies-literatureUnknown15Zhao XBacterial canker is a devastating disease in kiwifruit production, primarily caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>. In this study, a strain of <i>Bacillus velezensis</i> named JIN4, isolated from a kiwifruit branch, showed antagonistic activity. The <i>B</i>. <i>velezensis</i> JIN4 strain was identified based on its morphological, physiological and biochemical characteristics, 16S rDNA, and <i>gyrA</i> sequences. Furthermore, the complete genome of the strain was elucidated, revealing the presence of three genes that confer resistance to antibiotics, including tetracycline, lincomycin, and streptomycin. Additionally, a cluster of nine secondary metabolite synthesis genes was identified. In the laboratory, the JIN4 strain exhibited nitrogen (N) and phosphorus (P) produFrontiers in plant science1513438https://www.ebi.ac.uk/biostudies/studies/S-EPMC11701161biostudies-literatureIsolation and identification of a novel &lt;i&gt;Bacillus velezensis&lt;/i&gt; strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by &lt;i&gt;Pseudomonas syringae&lt;/i&gt; pv. &lt;i&gt;actinidiae&lt;/i&gt;.PMC11701161Yang YYi YZhai YQiu CZeng LWang FWang HWei LZhao XXia FfalseIsolation and identification of a novel &lt;i&gt;Bacillus velezensis&lt;/i&gt; strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by &lt;i&gt;Pseudomonas syringae&lt;/i&gt; pv. &lt;i&gt;actinidiae&lt;/i&gt;.Bacterial canker is a devastating disease in kiwifruit production, primarily caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>. In this study, a strain of <i>Bacillus velezensis</i> named JIN4, isolated from a kiwifruit branch, showed antagonistic activity. The <i>B</i>. <i>velezensis</i> JIN4 strain was identified based on its morphological, physiological and biochemical characteristics, 16S rDNA, and <i>gyrA</i> sequences. Furthermore, the complete genome of the strain was elucidated, revealing the presence of three genes that confer resistance to antibiotics, including tetracycline, lincomycin, and streptomycin. Additionally, a cluster of nine secondary metabolite synthesis genes was identified. In the laboratory, the JIN4 strain exhibited nitrogen (N) and phosphorus (P) produ2024-01-01T00:00:00Z20242026-05-02T14:29:54.728Z2026-04-07T18:06:37.721ZS-EPMC117011613976422710.3389/fpls.2024.1513438