{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["75(1)"],"submitter":["Iversen XES"],"funding":["Danmarks Frie Forskningsfond"],"pubmed_abstract":["Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S <i>rrs</i>, internal transcribed spacer and 23S <i>rrl</i> elements, as well as a single-copy core-gene (<i>hsp65</i>, <i>rpoB+C</i>, <i>secA</i> and <i>tuf</i>) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and <i>in silico</i> DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are <i>Mycobacterium wendilense</i> sp. nov. (Mu0050<sup>T</sup>=ITM 501390<sup>T</sup>=CCUG 77525<sup>T</sup>), <i>Mycobacterium burgundiense</i> sp. nov. (Mu0053<sup>T</sup>=ITM 501391<sup>T</sup>=CCUG 77526<sup>T</sup>), <i>Mycobacterium kokjensenii</i> sp. nov. (Mu0083<sup>T</sup>=ITM 501392<sup>T</sup>=CCUG 77527<sup>T</sup>) and <i>Mycobacterium holstebronense</i> sp. nov. (Mu0102<sup>T</sup>=ITM 501393<sup>T</sup>=CCUG 77528<sup>T</sup>)."],"journal":["International journal of systematic and evolutionary microbiology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11706282"],"repository":["biostudies-literature"],"pubmed_title":["Four novel nontuberculous mycobacteria species: <i>Mycobacterium wendilense</i> sp. nov., <i>Mycobacterium burgundiense</i> sp. nov., <i>Mycobacterium kokjensenii</i> sp. nov. and <i>Mycobacterium holstebronense</i> sp. nov. revived from a historical Danish strain collection."],"pmcid":["PMC11706282"],"funding_grant_id":["3127-00035B"],"pubmed_authors":["Iversen XES","Svensson E","Rasmussen EM","Lillebaek T","Meehan CJ","Folkvardsen DB","Jorgensen R","Norman A"],"additional_accession":[]},"is_claimable":false,"name":"Four novel nontuberculous mycobacteria species: <i>Mycobacterium wendilense</i> sp. nov., <i>Mycobacterium burgundiense</i> sp. nov., <i>Mycobacterium kokjensenii</i> sp. nov. and <i>Mycobacterium holstebronense</i> sp. nov. revived from a historical Danish strain collection.","description":"Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S <i>rrs</i>, internal transcribed spacer and 23S <i>rrl</i> elements, as well as a single-copy core-gene (<i>hsp65</i>, <i>rpoB+C</i>, <i>secA</i> and <i>tuf</i>) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and <i>in silico</i> DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are <i>Mycobacterium wendilense</i> sp. nov. (Mu0050<sup>T</sup>=ITM 501390<sup>T</sup>=CCUG 77525<sup>T</sup>), <i>Mycobacterium burgundiense</i> sp. nov. (Mu0053<sup>T</sup>=ITM 501391<sup>T</sup>=CCUG 77526<sup>T</sup>), <i>Mycobacterium kokjensenii</i> sp. nov. (Mu0083<sup>T</sup>=ITM 501392<sup>T</sup>=CCUG 77527<sup>T</sup>) and <i>Mycobacterium holstebronense</i> sp. nov. (Mu0102<sup>T</sup>=ITM 501393<sup>T</sup>=CCUG 77528<sup>T</sup>).","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-01T10:27:40.18Z","creation":"2026-04-08T11:29:28.857Z"},"accession":"S-EPMC11706282","cross_references":{"pubmed":["39773688"],"doi":["10.1099/ijsem.0.006620"]}}