biostudies-literatureCheng ENatural Sciences and Engineering Research Council of CanadaFonds de recherche du Québec – Nature et technologiesNIH HHSe1011351https://www.ebi.ac.uk/biostudies/studies/S-EPMC11706408biostudies-literatureUnknown20(12)Stem and progenitor cell mitosis is essential for tissue development and homeostasis. How these cells ensure proper chromosome segregation, and thereby maintain mitotic fidelity, in the complex physiological environment of a living animal is poorly understood. Here we use in situ live-cell imaging of C. elegans germline stem and progenitor cells (GSPCs) to ask how the signaling environment influences stem and progenitor cell mitosis in vivo. Through a candidate screen we identify a new role for the insulin/IGF receptor (IGFR), daf-2, during GSPC mitosis. Mitosis is delayed in daf-2/IGFR mutants, and these delays require canonical, DAF-2/IGFR to DAF-16/FoxO insulin signaling, here acting cell non-autonomously from the soma. Interestingly, mitotic delays in daf-2/IGFR mutants depend on the sPLoS geneticsNon-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells.PMC11706408RGPIN/05199-2020P40 OD010440283252Cheng EGerhold ARLu RfalseNon-autonomous insulin signaling delays mitotic progression in C. elegans germline stem and progenitor cells.Stem and progenitor cell mitosis is essential for tissue development and homeostasis. How these cells ensure proper chromosome segregation, and thereby maintain mitotic fidelity, in the complex physiological environment of a living animal is poorly understood. Here we use in situ live-cell imaging of C. elegans germline stem and progenitor cells (GSPCs) to ask how the signaling environment influences stem and progenitor cell mitosis in vivo. Through a candidate screen we identify a new role for the insulin/IGF receptor (IGFR), daf-2, during GSPC mitosis. Mitosis is delayed in daf-2/IGFR mutants, and these delays require canonical, DAF-2/IGFR to DAF-16/FoxO insulin signaling, here acting cell non-autonomously from the soma. Interestingly, mitotic delays in daf-2/IGFR mutants depend on the s2024-01-01T00:00:00Z2024 Dec2026-05-29T15:22:52.544Z2025-04-04T21:49:29.543ZS-EPMC117064083971526910.1371/journal.pgen.1011351