biostudies-literatureLiehr TDeutsche ForschungsgemeinschaftInterdisciplinary Centre for Clinical Research within the Faculty of Medicine at the RWTH Aachen UniversityDeutsche Krebshilfe21https://www.ebi.ac.uk/biostudies/studies/S-EPMC11719652biostudies-literatureUnknown14(1)The Rat-1 cell line was established as a subclone of the parental rat fibroblastoid line F2408, derived from Fisher 344 rat embryos. Rat-1 cells are widely used in various research fields, especially in cancer biology, to study the effects of oncogenes on cell proliferation. They are also crucial for investigating signal transduction pathways and play a key role in drug testing and pharmacological studies due to their rapid proliferation. Therefore, Rat-1 cells are an indispensable research tool. While some cytogenetic information on their basic chromosomal features is available, detailed genomic analyses, such as karyotype analysis, short tandem repeat (STR) profiling, and whole-genome sequencing, have not been thoroughly conducted. As a result, the genetic stability and potential variatiCellsGenetic Characteristics of the Rat Fibroblast Cell Line Rat-1.PMC1171965270115581WE2554/13-1, WE2554/15-1, and WE2554/17-1PTD 1-5Liehr TKankel SSchroder-Lange SKWeiskirchen RBuhl EMfalseGenetic Characteristics of the Rat Fibroblast Cell Line Rat-1.The Rat-1 cell line was established as a subclone of the parental rat fibroblastoid line F2408, derived from Fisher 344 rat embryos. Rat-1 cells are widely used in various research fields, especially in cancer biology, to study the effects of oncogenes on cell proliferation. They are also crucial for investigating signal transduction pathways and play a key role in drug testing and pharmacological studies due to their rapid proliferation. Therefore, Rat-1 cells are an indispensable research tool. While some cytogenetic information on their basic chromosomal features is available, detailed genomic analyses, such as karyotype analysis, short tandem repeat (STR) profiling, and whole-genome sequencing, have not been thoroughly conducted. As a result, the genetic stability and potential variati2024-01-01T00:00:00Z2024 Dec2026-04-08T19:04:36.875Z2026-04-08T11:43:45.02ZS-EPMC117196523979172210.3390/cells14010021