biostudies-literatureCheng JInnovation Project of Guangxi Graduate EducationNatural Science Foundation of Guangxi ProvinceNational Natural Science Foundation of ChinaGuangxi Agricultural Science and Technology Self-funded projectNational Key Research and Development Program of China153https://www.ebi.ac.uk/biostudies/studies/S-EPMC11719787biostudies-literatureUnknown26(1)The specific expression profile and function of circular RNA (circRNA) in follicular atresia remain largely unknown. Here, the circRNA expression profiles of granulosa cells derived from healthy follicles (HFs) and antral follicles (AFs) in buffalo were analyzed by RNA-seq, and the mechanism of a differentially expressed circRNA (DEcircRNA) circTEC regulating the granulosa cell function that affects follicular atresia was further explored. RNA-seq results showed that a total of 112 DEcircRNAs were identified. Among them, circTEC was highly expressed in HF, and its circular structure was confirmed by RNase R digestion assay, reversed PCR and Sanger sequencing. Functional experiments demonstrated that circTEC promotes the proliferation and steroid hormone synthesis of buffalo granulosa cellsInternational journal of molecular sciencesCircTEC Inhibits the Follicular Atresia in Buffalo (<i>Bubalus bubalis</i>) via Targeting miR-144-5p/FZD3 Signaling Axis.PMC117197872023GXNSFAA026367323608312023YFD1300502YCBZ2022030Z2024128Pan YYang YShi DZhang RXing QCheng JDeng YfalseCircTEC Inhibits the Follicular Atresia in Buffalo (<i>Bubalus bubalis</i>) via Targeting miR-144-5p/FZD3 Signaling Axis.The specific expression profile and function of circular RNA (circRNA) in follicular atresia remain largely unknown. Here, the circRNA expression profiles of granulosa cells derived from healthy follicles (HFs) and antral follicles (AFs) in buffalo were analyzed by RNA-seq, and the mechanism of a differentially expressed circRNA (DEcircRNA) circTEC regulating the granulosa cell function that affects follicular atresia was further explored. RNA-seq results showed that a total of 112 DEcircRNAs were identified. Among them, circTEC was highly expressed in HF, and its circular structure was confirmed by RNase R digestion assay, reversed PCR and Sanger sequencing. Functional experiments demonstrated that circTEC promotes the proliferation and steroid hormone synthesis of buffalo granulosa cells2024-01-01T00:00:00Z2024 Dec2026-07-01T03:23:54.309Z2026-07-01T03:12:32.244ZS-EPMC117197873979601510.3390/ijms26010153