biostudies-literatureVerma SMinisterstvo Školství, Mládeže a TělovýchovyMasaryk UniversityH2020 Spreading Excellence and Widening Participation6https://www.ebi.ac.uk/biostudies/studies/S-EPMC11723901biostudies-literatureUnknown38(1)<h4>Key message</h4>SHATTERPROOF 2 regulates TAA1 expression for the establishment of the gynoecium valve margins. Gynoecium development and patterning play a crucial role in determining the ultimate structure of the fruit and, thus, seed production. The MADS-box transcription factor SHATTERPROOF 2 (SHP2) contributes to valve margin differentiation and plays a major role in fruit dehiscence and seed dispersal. Despite the acknowledged contribution of auxin to gynoecium development, its precise role in valve margin establishment remains somewhat enigmatic. Our study addresses this gap by uncovering the role of SHP2 as a positive regulator of key auxin biosynthetic genes, TAA1 and YUCCA 4. Genetic and molecular analyses revealed that SHP2 directly regulates the expression of TAA1 in the valvPlant reproductionThe MADS-box protein SHATTERPROOF 2 regulates TAA1 expression in the gynoecium valve margins.PMC11723901895296CZ.02.1.01/0.0/0.0/16_019/0000738Verma SSvihlova LRobert HSfalseThe MADS-box protein SHATTERPROOF 2 regulates TAA1 expression in the gynoecium valve margins.<h4>Key message</h4>SHATTERPROOF 2 regulates TAA1 expression for the establishment of the gynoecium valve margins. Gynoecium development and patterning play a crucial role in determining the ultimate structure of the fruit and, thus, seed production. The MADS-box transcription factor SHATTERPROOF 2 (SHP2) contributes to valve margin differentiation and plays a major role in fruit dehiscence and seed dispersal. Despite the acknowledged contribution of auxin to gynoecium development, its precise role in valve margin establishment remains somewhat enigmatic. Our study addresses this gap by uncovering the role of SHP2 as a positive regulator of key auxin biosynthetic genes, TAA1 and YUCCA 4. Genetic and molecular analyses revealed that SHP2 directly regulates the expression of TAA1 in the valv2025-01-01T00:00:00Z2025 Jan2025-04-22T04:40:46.407Z2025-04-05T21:05:26.666ZS-EPMC117239013979229810.1007/s00497-024-00518-6