{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Solberg T"],"funding":["Swiss National Science Foundation","European Research Council","World Premier International Research Center Initiative (WPI), MEXT, Japan","National Center of Competence in Research (NCCR) RNA and Disease"],"pagination":["273-296"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11723920"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["Facultative heterochromatin is marked by the repressive histone modification H3K27me3 in eukaryotes. Deposited by the PRC2 complex, H3K27me3 is essential for regulating gene expression during development, and chromatin bearing this mark is generally considered transcriptionally inert. The PRC2 complex has also been linked to programmed DNA elimination during development in ciliates such as Paramecium. Due to a lack of mechanistic insight, a direct involvement has been questioned as most eliminated DNA segments in Paramecium are shorter than the size of a nucleosome. Here, we identify two sets of histone methylation readers essential for PRC2-mediated DNA elimination in Paramecium: Firefly1/2 and Mayfly1-4. The chromodomain proteins Firefly1/2 act in tight association with TFIIS4, a transcr"],"journal":["EMBO reports"],"pubmed_title":["Heterochromatin-dependent transcription links the PRC2 complex to small RNA-mediated DNA elimination."],"pmcid":["PMC11723920"],"funding_grant_id":["146257","681178","31003A_166407","31003A_146257","260358"],"pubmed_authors":["Wen Z","Matsubara R","Wang C","Solberg T","Nowacki M"],"additional_accession":[]},"is_claimable":false,"name":"Heterochromatin-dependent transcription links the PRC2 complex to small RNA-mediated DNA elimination.","description":"Facultative heterochromatin is marked by the repressive histone modification H3K27me3 in eukaryotes. Deposited by the PRC2 complex, H3K27me3 is essential for regulating gene expression during development, and chromatin bearing this mark is generally considered transcriptionally inert. The PRC2 complex has also been linked to programmed DNA elimination during development in ciliates such as Paramecium. Due to a lack of mechanistic insight, a direct involvement has been questioned as most eliminated DNA segments in Paramecium are shorter than the size of a nucleosome. Here, we identify two sets of histone methylation readers essential for PRC2-mediated DNA elimination in Paramecium: Firefly1/2 and Mayfly1-4. The chromodomain proteins Firefly1/2 act in tight association with TFIIS4, a transcr","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-01T15:07:16.081Z","creation":"2026-04-08T13:33:26.726Z"},"accession":"S-EPMC11723920","cross_references":{"pubmed":["39614125"],"doi":["10.1038/s44319-024-00332-1"]}}