{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Park SJ"],"funding":["National Research Foundation of Korea (NRF)","Chung Yang, Cha Young Sun & Jang Hi Joo Memorial Fund","National Research Foundation of Korea"],"pagination":["677"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11733285"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.6 times smaller than SpCas9, providing advantages for gene therapy research. Despite extensive engineering efforts to improve Un1Cas12f1, the editing efficiency of Un1Cas12f1 is still shown to be low depending on the target site. To overcome this limitation, we develop enhanced Cas12f1 (eCas12f1), which exhibits gene editing activity similar to SpCas9 and AsCpf1, even in gene targets where prev"],"journal":["Nature communications"],"pubmed_title":["Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1."],"pmcid":["PMC11733285"],"funding_grant_id":["RS-2023-00220894"],"pubmed_authors":["Yang J","Kim K","Kim HS","Lee H","Jeong TY","Choi J","Park SJ","Ju S","Jung WJ","Yoon DE","Lee JH"],"additional_accession":[]},"is_claimable":false,"name":"Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1.","description":"With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.6 times smaller than SpCas9, providing advantages for gene therapy research. Despite extensive engineering efforts to improve Un1Cas12f1, the editing efficiency of Un1Cas12f1 is still shown to be low depending on the target site. To overcome this limitation, we develop enhanced Cas12f1 (eCas12f1), which exhibits gene editing activity similar to SpCas9 and AsCpf1, even in gene targets where prev","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-02T10:12:11.484Z","creation":"2025-04-06T01:15:17.34Z"},"accession":"S-EPMC11733285","cross_references":{"pubmed":["39809780"],"doi":["10.1038/s41467-025-56048-w"]}}