{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["63(1)"],"submitter":["Trecourt A"],"funding":["Bourse de recherche PAM Biologie-ACP des Hospices Civils de Lyon 2022"],"pubmed_abstract":["Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum may present atypical histopathological features inducing diagnostic errors. We aimed to estimate the frequency of these atypical features in formalin-fixed tissue (FT) samples and to assess the relevance of an integrated histomolecular diagnosis using specific H. capsulatum PCR and panfungal PCR followed by Sanger sequencing and/or targeted massive parallel sequencing (MPS). A total of 27 FT from 23 patients with a histopathological diagnosis of cryptococcosis (n = 16 FT from 13 patients) or histoplasmosis (n = 11 FT from 10 patients) were retrospectively included. All FT were consultation cases. Mycological identifications on equivalent fresh tissue were available for 11/23 (47.8%) patients. The expert pathologist r"],"journal":["Medical mycology"],"pagination":["myae126"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11735191"],"repository":["biostudies-literature"],"pubmed_title":["Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum infections on tissue sections: Diagnostic pitfalls and relevance of an integrated histomolecular diagnosis."],"pmcid":["PMC11735191"],"pubmed_authors":["Wallon M","Baltres A","Menotti J","Trecourt A","Lemoine JP","Bentaher A","Dupont D","Alanio A","Chapey-Picq E","Miossec C","Persat F","Rabodonirina M","Donzel M","Devouassoux-Shisheboran M","Tirard-Collet P"],"additional_accession":[]},"is_claimable":false,"name":"Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum infections on tissue sections: Diagnostic pitfalls and relevance of an integrated histomolecular diagnosis.","description":"Cryptococcus neoformans/gattii and Histoplasma capsulatum var. capsulatum may present atypical histopathological features inducing diagnostic errors. We aimed to estimate the frequency of these atypical features in formalin-fixed tissue (FT) samples and to assess the relevance of an integrated histomolecular diagnosis using specific H. capsulatum PCR and panfungal PCR followed by Sanger sequencing and/or targeted massive parallel sequencing (MPS). A total of 27 FT from 23 patients with a histopathological diagnosis of cryptococcosis (n = 16 FT from 13 patients) or histoplasmosis (n = 11 FT from 10 patients) were retrospectively included. All FT were consultation cases. Mycological identifications on equivalent fresh tissue were available for 11/23 (47.8%) patients. The expert pathologist r","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2025-04-04T02:01:15.706Z","creation":"2025-04-04T02:01:15.706Z"},"accession":"S-EPMC11735191","cross_references":{"pubmed":["39732625"],"doi":["10.1093/mmy/myae126"]}}