{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hayward RF"],"funding":["NIMH NIH HHS","NINDS NIH HHS"],"pagination":["102983"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11735331"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["125"],"pubmed_abstract":["Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optical system for simultaneous targeted Ca2+ import and Ca2+ concentration mapping. We co-expressed a blue light-activated calcium selective channelrhodopsin, CapChR2, with a far-red calcium sensor, FR-GECO1c, in cultured rat hippocampal neurons, and used patterned optogenetic stimulation to introduce calcium into cells with user-defined patterns of space and time. We determined a mean steady-state length constant for Ca2+ transport ϕ ∼ 5.8 μm, a half-life for return to baseline t1/2 ∼ 1.7 s, and an effective diffusion coefficient D ∼ 20 μm2/s, though there were substantial differences in Ca2+ dynamics between proximal and distal dendrites. At high Ca2+ concentration, distal dendrites showed nonlinear activation of Ca2+ efflux, which we pharmacologically ascribed to the NCX1 antiporter. Genetically encoded tools for all-optical mapping of Ca2+ transport and handling provide a powerful capability for studying this important messenger."],"journal":["Cell calcium"],"pubmed_title":["All-optical mapping of Ca<sup>2+</sup> transport and homeostasis in dendrites."],"pmcid":["PMC11735331"],"funding_grant_id":["RF1 MH117042","RF1 NS126043"],"pubmed_authors":["Cohen AE","Hayward RF"],"additional_accession":[]},"is_claimable":false,"name":"All-optical mapping of Ca<sup>2+</sup> transport and homeostasis in dendrites.","description":"Calcium mediates many important signals in dendrites. However, the basic transport properties of calcium in dendrites have been difficult to measure: how far and how fast does a local influx of calcium propagate? We developed an all-optical system for simultaneous targeted Ca2+ import and Ca2+ concentration mapping. We co-expressed a blue light-activated calcium selective channelrhodopsin, CapChR2, with a far-red calcium sensor, FR-GECO1c, in cultured rat hippocampal neurons, and used patterned optogenetic stimulation to introduce calcium into cells with user-defined patterns of space and time. We determined a mean steady-state length constant for Ca2+ transport ϕ ∼ 5.8 μm, a half-life for return to baseline t1/2 ∼ 1.7 s, and an effective diffusion coefficient D ∼ 20 μm2/s, though there were substantial differences in Ca2+ dynamics between proximal and distal dendrites. At high Ca2+ concentration, distal dendrites showed nonlinear activation of Ca2+ efflux, which we pharmacologically ascribed to the NCX1 antiporter. Genetically encoded tools for all-optical mapping of Ca2+ transport and handling provide a powerful capability for studying this important messenger.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-06T08:44:30.383Z","creation":"2026-05-28T03:12:10.197Z"},"accession":"S-EPMC11735331","cross_references":{"pubmed":["39662137"],"doi":["10.1016/j.ceca.2024.102983"]}}