biostudies-literatureTian ZScience and Technological Project of China National Tobacco CorporationThe Agricultural Science and Technology Innovation ProgramScience and Technological Project of China Tobacco Jiangsu Industrial Co., LtdFundamental Research Funds for Central Non-profit Scientific Institution2451700https://www.ebi.ac.uk/biostudies/studies/S-EPMC11740682biostudies-literatureUnknown20(1)Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting of three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles in the embryonic development, photosynthesis, flowering, abiotic stress responses, and other essential processes in plants. To better understand the genome-wide NF-Y domain-containing proteins, the protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter <i>cis</i>-elements, and protein interaction network of NtNF-Ys in tobacco (<i>Nicotiana tabacum</i> L.) were systematically analyzed. In this study, we identified 58 NtNF-Ys in tobacco, respectively, and divided into three subfamilies corresponding to their phylogenetic relatioPlant signaling & behaviorGenome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco (&lt;i&gt;Nicotiana tabacum&lt;/i&gt; L.).PMC11740682110202103015H202110ASTIP-TRIC021610232024009Tian ZCheng FLi SWang BChao JWang DFu JXue LSong WYue XSun JMao JfalseGenome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco (&lt;i&gt;Nicotiana tabacum&lt;/i&gt; L.).Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting of three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles in the embryonic development, photosynthesis, flowering, abiotic stress responses, and other essential processes in plants. To better understand the genome-wide NF-Y domain-containing proteins, the protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter <i>cis</i>-elements, and protein interaction network of NtNF-Ys in tobacco (<i>Nicotiana tabacum</i> L.) were systematically analyzed. In this study, we identified 58 NtNF-Ys in tobacco, respectively, and divided into three subfamilies corresponding to their phylogenetic relatio2025-01-01T00:00:00Z2025 Dec2026-06-02T01:50:44.873Z2026-04-13T03:12:35.202ZS-EPMC117406823981766210.1080/15592324.2025.2451700