biostudies-literatureLiu YChengdu University of Traditional Chinese Medicine "Xinglin Scholars" Discipline Talent Research Enhancement Program Hospital Special ProjectSichuan Province Traditional Chinese Medicine Management Traditional Chinese Medicine Research Special ProjectNational Natural Science Foundation of ChinaThe Medical Research Projects in Chengdu Citye70282https://www.ebi.ac.uk/biostudies/studies/S-EPMC11740985biostudies-literatureUnknown29(2)Hepatocellular carcinoma (HCC) is a malignant tumour that poses a serious threat to human health and places a heavy burden on individuals and society. However, the role of GPC1 in the malignant progression of HCC is unknown. In this study, we analysed the expression of GPC1 in HCC, and its association with poor patient prognosis. The effects of GPC1 on the proliferation, invasion and migration of HCC were analysed through cellular functional experiments in vitro and in vivo. Mechanistically, DNA methylation of GPC1 was analysed by DNA extraction, methylation-specific PCR and bisulfite Sanger sequencing (BSP), and the target genes TET1 and miRNA regulating DNA methylation of GPC1 were found through the bioinformatics database. The results revealed that GPC1 was highly expressed in HCC, and Journal of cellular and molecular medicinemiR-143-3p/TET1 Axis Regulates GPC1 Through DNA Methylation and Impairs the Malignant Biological Behaviour of HCC via the Hippo Signalling Pathway.PMC11740985821006042023MS322202227582003828YYZX2021035Liu YGu XDu DXiong BHe QfalsemiR-143-3p/TET1 Axis Regulates GPC1 Through DNA Methylation and Impairs the Malignant Biological Behaviour of HCC via the Hippo Signalling Pathway.Hepatocellular carcinoma (HCC) is a malignant tumour that poses a serious threat to human health and places a heavy burden on individuals and society. However, the role of GPC1 in the malignant progression of HCC is unknown. In this study, we analysed the expression of GPC1 in HCC, and its association with poor patient prognosis. The effects of GPC1 on the proliferation, invasion and migration of HCC were analysed through cellular functional experiments in vitro and in vivo. Mechanistically, DNA methylation of GPC1 was analysed by DNA extraction, methylation-specific PCR and bisulfite Sanger sequencing (BSP), and the target genes TET1 and miRNA regulating DNA methylation of GPC1 were found through the bioinformatics database. The results revealed that GPC1 was highly expressed in HCC, and 2025-01-01T00:00:00Z2025 Jan2026-05-02T14:27:53.441Z2025-04-07T04:19:03.59ZS-EPMC117409853982326810.1111/jcmm.70282