biostudies-literatureUnknownOsei EKNIGMS NIH HHSBacteriophage research has experienced a renaissance in recent years, owing to their therapeutic potential and versatility in biotechnology, particularly in combating antibiotic resistant-bacteria along the farm-to-fork continuum. However, certain pathogens remain underexplored as targets for phage therapy, including the zoonotic pathogen <i>Streptococcus suis</i> which causes infections in pigs and humans. Despite global efforts, the genome of only one infective <i>S. suis</i> phage has been described. Here, we report the isolation of two phages that infect <i>S. suis</i>: Bonnie and Clyde. The phages infect 58% of 100 <i>S. suis</i> strains tested, including representatives of seven different serotypes and thirteen known sequence types from diverse geographical origins. Clyde suppressed bioRxiv : the preprint server for biology2025.01.07.631744https://www.ebi.ac.uk/biostudies/studies/S-EPMC11741397biostudies-literatureIsolation of phages infecting the zoonotic pathogen <i>Streptococcus suis</i> reveals novel structural and genomic characteristics.PMC11741397R01 GM129325O'Hea RFranz CMAPO'Doherty AAthalye AWilson MMahony JWeinert LACambillau CKenny JGOsei EKHille FManzanilla EGMurray GGRfalseIsolation of phages infecting the zoonotic pathogen <i>Streptococcus suis</i> reveals novel structural and genomic characteristics.Bacteriophage research has experienced a renaissance in recent years, owing to their therapeutic potential and versatility in biotechnology, particularly in combating antibiotic resistant-bacteria along the farm-to-fork continuum. However, certain pathogens remain underexplored as targets for phage therapy, including the zoonotic pathogen <i>Streptococcus suis</i> which causes infections in pigs and humans. Despite global efforts, the genome of only one infective <i>S. suis</i> phage has been described. Here, we report the isolation of two phages that infect <i>S. suis</i>: Bonnie and Clyde. The phages infect 58% of 100 <i>S. suis</i> strains tested, including representatives of seven different serotypes and thirteen known sequence types from diverse geographical origins. Clyde suppressed 2025-01-01T00:00:00Z2025 Jan2026-04-07T21:22:55.121Z2025-04-05T21:20:35.264ZS-EPMC117413973982974610.1101/2025.01.07.631744