{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bdzhola A"],"funding":["National Research Foundation of Ukraine","FEBS Collaborative Developmental Scholarship","British Academy of Science Fellowship","The Biotechnology and Biological Sciences Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["130"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11742003"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["52(1)"],"pubmed_abstract":["Background
Ribosomal protein S6 kinase 1 (p70S6K1) is a member of the AGC family of serine/threonine kinases which plays a role in various cellular processes, including protein synthesis, cell growth, and survival. Dysregulation of p70S6K1, characterized by its overexpression and/or hyperactivation, has been implicated in numerous human pathologies, particularly in several types of cancer. Therefore, generating active, recombinant p70S6K1 is critical for investigating its role in cancer biology and for developing novel diagnostic or therapeutic approaches.Methods
The baculovirus dual expression system was utilized, enabling the co-expression of two recombinant proteins in infected cells: (a) His-tagged S6K1 with a deletion of the C-terminal autoinhibitory motif and a phosph"],"journal":["Molecular biology reports"],"pubmed_title":["Co-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using bac-to-bac baculovirus expression system."],"pmcid":["PMC11742003"],"funding_grant_id":["BB/S009027/1","BB/L010410/1","RaR\\100514","2023.03/0120"],"pubmed_authors":["Zhyvoloup A","Malanchuk O","Gout I","Filonenko V","Bdzhola A","Palchevskyi S"],"additional_accession":[]},"is_claimable":false,"name":"Co-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using bac-to-bac baculovirus expression system.","description":"Background
Ribosomal protein S6 kinase 1 (p70S6K1) is a member of the AGC family of serine/threonine kinases which plays a role in various cellular processes, including protein synthesis, cell growth, and survival. Dysregulation of p70S6K1, characterized by its overexpression and/or hyperactivation, has been implicated in numerous human pathologies, particularly in several types of cancer. Therefore, generating active, recombinant p70S6K1 is critical for investigating its role in cancer biology and for developing novel diagnostic or therapeutic approaches.Methods
The baculovirus dual expression system was utilized, enabling the co-expression of two recombinant proteins in infected cells: (a) His-tagged S6K1 with a deletion of the C-terminal autoinhibitory motif and a phosph","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-06T17:41:46.142Z","creation":"2025-04-05T21:20:16.73Z"},"accession":"S-EPMC11742003","cross_references":{"pubmed":["39821712"],"doi":["10.1007/s11033-024-10136-0"]}}