biostudies-literatureIto MA.P. Giannini FoundationMEXT | Japan Society for the Promotion of Science (JSPS)Howard Hughes Medical Institute (HHMI)e2412961121https://www.ebi.ac.uk/biostudies/studies/S-EPMC11745341biostudies-literatureUnknown122(2)During meiosis, each pair of homologous chromosomes becomes connected by at least one crossover, as required for accurate segregation, and adjacent crossovers are widely separated thereby limiting total numbers. In coarsening models, this crossover patterning results from nascent recombination sites competing to accrue a limiting pro-crossover RING-domain protein (COR) that diffuses between synapsed chromosomes. Here, we delineate the localization dynamics of three mammalian CORs in the mouse and determine their interdependencies. RNF212, HEI10, and the newest member RNF212B show divergent spatiotemporal dynamics along synapsed chromosomes, including profound differences in spermatocytes and oocytes, that are not easily reconciled by elementary coarsening models. Contrasting mutant phenotyProceedings of the National Academy of Sciences of the United States of AmericaDistinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis.PMC11745341EXROP fellowshipPostdocroral FellowshipInvestigatorpostdoctoral fellowshipLee SSandhu SHuang CHuang ILee KIto MKulkarni DSHirota RMPrendergast RNunez BYun YHu LHunter NLim NfalseDistinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis.During meiosis, each pair of homologous chromosomes becomes connected by at least one crossover, as required for accurate segregation, and adjacent crossovers are widely separated thereby limiting total numbers. In coarsening models, this crossover patterning results from nascent recombination sites competing to accrue a limiting pro-crossover RING-domain protein (COR) that diffuses between synapsed chromosomes. Here, we delineate the localization dynamics of three mammalian CORs in the mouse and determine their interdependencies. RNF212, HEI10, and the newest member RNF212B show divergent spatiotemporal dynamics along synapsed chromosomes, including profound differences in spermatocytes and oocytes, that are not easily reconciled by elementary coarsening models. Contrasting mutant phenoty2025-01-01T00:00:00Z2025 Jan2026-05-29T14:34:10.424Z2025-04-05T09:32:29.834ZS-EPMC117453413976140210.1073/pnas.2412961121