biostudies-literatureUnknown15Correa Lopes B<h4>Introduction</h4>Fecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial viability is unknown. We aimed (1) to assess the viability of several core intestinal bacterial species by qPCR and (2) to assess <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> viability through culture to further characterize bacterial viability in different protocols for FMT preparations.<h4>Methods</h4>Bacterial abundances were assessed in feces from six healthy dogs by qPCR after propidium monoazide (PMA-qPCR) treatment for selective quantitation of viable bacteria. Conservation methods tested included lyophilizaFrontiers in microbiology1502452https://www.ebi.ac.uk/biostudies/studies/S-EPMC11747423biostudies-literatureProlonged storage reduces viability of &lt;i&gt;Peptacetobacter (Clostridium) hiranonis&lt;/i&gt; and core intestinal bacteria in fecal microbiota transplantation preparations for dogs.PMC11747423Turck JTolbert MKGiaretta PRPilla RCorrea Lopes BSuchodolski JSfalseProlonged storage reduces viability of &lt;i&gt;Peptacetobacter (Clostridium) hiranonis&lt;/i&gt; and core intestinal bacteria in fecal microbiota transplantation preparations for dogs.<h4>Introduction</h4>Fecal microbiota transplantation (FMT) has been described useful as an adjunct treatment for chronic enteropathy in dogs. Different protocols can be used to prepare and store FMT preparations, however, the effect of these methods on microbial viability is unknown. We aimed (1) to assess the viability of several core intestinal bacterial species by qPCR and (2) to assess <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> viability through culture to further characterize bacterial viability in different protocols for FMT preparations.<h4>Methods</h4>Bacterial abundances were assessed in feces from six healthy dogs by qPCR after propidium monoazide (PMA-qPCR) treatment for selective quantitation of viable bacteria. Conservation methods tested included lyophiliza2024-01-01T00:00:00Z20242025-04-04T20:56:00.666Z2025-04-04T20:56:00.666ZS-EPMC117474233983910510.3389/fmicb.2024.1502452