biostudies-literatureUnknown15(1)Krishnamurthy HKTick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and transmit several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping signs and symptoms of tick-borne infections can often lead to misdiagnosis affecting treatment and the prevalence data reported especially for non-Lyme tick-borne infections. The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple pathScientific reports2527https://www.ebi.ac.uk/biostudies/studies/S-EPMC11747503biostudies-literatureA customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases.PMC11747503Bei KRajasekaran JJKrishnamurthy HKHedman KWelc-Faleciak RBlanton LSAbdallah MAAChangalath CD'Angelo PRuzek DAbu-Raddadi LJFornara CFurione MPsaroulaki AMatilda SFortova AJayaraman VWang TNtoula AKrishna KLilleri DChrdle APawelczyk AAl-Sadeq DWNasrallah GKEscarcega-Avila ASoderlund-Venermo MMakridaki EChochlakis DRai AJfalseA customizable multiplex protein microarray for antibody testing and its application for tick-borne and other infectious diseases.Tick-borne infections are the most common vector-borne diseases in the USA. Ticks harbor and transmit several infections with Lyme disease being the most common tickborne infection in the US and Europe. Lack of awareness about tick populations, specific diagnostic tests, and overlapping signs and symptoms of tick-borne infections can often lead to misdiagnosis affecting treatment and the prevalence data reported especially for non-Lyme tick-borne infections. The diagnostic tests currently available for tick-borne diseases are severely limited in their ability to provide accurate results and cannot detect multiple pathogens in a single run. The multiplex protein microarray developed at Vibrant was designed to detect multiple serological antibodies thereby detecting exposure to multiple path2025-01-01T00:00:00Z2025 Jan2025-04-04T20:56:04.21Z2025-04-04T20:56:04.21ZS-EPMC117475033983319610.1038/s41598-024-84467-0