biostudies-literatureUnknown16(1)Castillo-Lopez E<h4>Background</h4>The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified through cluster analysis and were identified as poor, average, and excellent quality. The proteome was assessed with quantitative shotgun proteomics; abundance data were compared among the colostrum types; enrichment analysis of metabolic processes and proteins classes was performed as well. We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.<h4>Results</h4>On average, 428 proteins were identified per sample, whJournal of animal science and biotechnology10https://www.ebi.ac.uk/biostudies/studies/S-EPMC11748342biostudies-literatureCharacterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations.PMC11748342Zebeli QHummel KReisinger NBiber PKreuzer-Redmer SSener-Aydemir ARazzazi-Fazeli ECastillo-Lopez EHartinger TfalseCharacterization of the colostrum proteome of primiparous Holstein cows and its association with colostrum immunoglobulin G concentrations.<h4>Background</h4>The objective was to characterize the colostrum proteome of primiparous Holstein cows in association with immunoglobulin G (IgG) content. Immediately after calving, colostrum samples were collected from 18 cows to measure IgG concentration. Based on colostrum IgG content, samples were classified through cluster analysis and were identified as poor, average, and excellent quality. The proteome was assessed with quantitative shotgun proteomics; abundance data were compared among the colostrum types; enrichment analysis of metabolic processes and proteins classes was performed as well. We also tested correlations between this proteome and blood globulin level of cows and passive immunity level of calves.<h4>Results</h4>On average, 428 proteins were identified per sample, wh2025-01-01T00:00:00Z2025 Jan2026-06-02T10:22:13.531Z2025-04-05T23:36:28.087ZS-EPMC117483423983397810.1186/s40104-024-01144-y