biostudies-literatureUnknown1(3)Li HVogt-Koyanagi-Harada (VKH) disease is a systemic autoimmune disorder threatening the eyesight. The pathogenic mechanisms and biomarkers reflecting disease severity and predicting treatment response require further exploration. Here, we performed a single-cell analysis of peripheral blood mononuclear cells (PBMC) obtained from eight patients with VKH disease and eight healthy controls to comprehensively delineate the changes in VKH disease. We showed a mixture of inflammation, effector, and exhausted states for PBMCs in VKH disease. Notably, our study implicated a newly identified B cell subset, natural killer-like B cells (K-BC) characterized by expressing CD19 and CD56, was correlated with VKH disease. K-BCs expanded in VKH disease, fell back after effective treatment, and promoted the diLife medicine387-400https://www.ebi.ac.uk/biostudies/studies/S-EPMC11749541biostudies-literaturePeripheral immune landscape and natural killer-like B cells in human Vogt-Koyanagi-Harada disease.PMC11749541Liu XLi HZheng YLi ZWang RHuang ZZhu LXie LYang SChen BSu WYe JfalsePeripheral immune landscape and natural killer-like B cells in human Vogt-Koyanagi-Harada disease.Vogt-Koyanagi-Harada (VKH) disease is a systemic autoimmune disorder threatening the eyesight. The pathogenic mechanisms and biomarkers reflecting disease severity and predicting treatment response require further exploration. Here, we performed a single-cell analysis of peripheral blood mononuclear cells (PBMC) obtained from eight patients with VKH disease and eight healthy controls to comprehensively delineate the changes in VKH disease. We showed a mixture of inflammation, effector, and exhausted states for PBMCs in VKH disease. Notably, our study implicated a newly identified B cell subset, natural killer-like B cells (K-BC) characterized by expressing CD19 and CD56, was correlated with VKH disease. K-BCs expanded in VKH disease, fell back after effective treatment, and promoted the di2022-01-01T00:00:00Z2022 Dec2025-04-05T11:34:17.346Z2025-04-05T11:34:17.346ZS-EPMC117495413987274710.1093/lifemedi/lnac047