biostudies-literatureUnknown2(6)Zhou DThe pathogenesis of several kidney diseases results in the eventual destruction of the renal tubular system, which can progress to end-stage renal disease. Previous studies have demonstrated the involvement of a population of SOX9-positive cells in kidney regeneration and repair process following kidney injury. However, the ability of these cells to autonomously generate kidney organoids has never been investigated. Here, we isolated SOX9+ kidney progenitor cells (KPCs) from both mice and humans and tested their differentiation potential in vitro. The data showed that the human SOX9+ KPC could self-assemble into organoids with kidney-like morphology. We also used single-cell RNA sequencing to characterize the organoid cell populations and identified four distinLife medicinelnad047https://www.ebi.ac.uk/biostudies/studies/S-EPMC11749593biostudies-literatureGeneration of renal tubular organoids from adult SOX9+ kidney progenitor cells.PMC11749593Duan JZuo WLi DZhou DLiu SWang YNie HfalseGeneration of renal tubular organoids from adult SOX9+ kidney progenitor cells.The pathogenesis of several kidney diseases results in the eventual destruction of the renal tubular system, which can progress to end-stage renal disease. Previous studies have demonstrated the involvement of a population of SOX9-positive cells in kidney regeneration and repair process following kidney injury. However, the ability of these cells to autonomously generate kidney organoids has never been investigated. Here, we isolated SOX9+ kidney progenitor cells (KPCs) from both mice and humans and tested their differentiation potential in vitro. The data showed that the human SOX9+ KPC could self-assemble into organoids with kidney-like morphology. We also used single-cell RNA sequencing to characterize the organoid cell populations and identified four distin2023-01-01T00:00:00Z2023 Dec2025-04-21T23:49:41.548Z2025-04-05T19:21:41.834ZS-EPMC117495933987205810.1093/lifemedi/lnad047