{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wyatt NJ"],"funding":["Wellcome Trust"],"pagination":["105550"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11751561"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["112"],"pubmed_abstract":["<h4>Background</h4>Large-scale multicentre studies are needed to understand complex relationships between the gut microbiota, health and disease. Interrogating the mucosal microbiota may identify important biology not captured by stool analysis. Gold standard tissue cryopreservation ('flash freezing') limits large-scale study feasibility. We aimed to compare gut microbiota in gold standard and pragmatic mucosal biopsy storage conditions.<h4>Methods</h4>We collected endoscopic recto-sigmoid biopsies from 20 adults with inflammatory bowel disease. Biopsies were preserved using three methods: (i) flash freezing (most proximal and distal biopsy sites); (ii) nucleic acid preservative reagents (QIAGEN Allprotect®, Invitrogen RNAlater™, and Zymo DNA/RNA Shield™); and (iii) formalin fixation with "],"journal":["EBioMedicine"],"pubmed_title":["Evaluation of intestinal biopsy tissue preservation methods to facilitate large-scale mucosal microbiota research."],"pmcid":["PMC11751561"],"funding_grant_id":["225875/Z/22/Z","208750/Z/17/Z","221745/Z/20/Z"],"pubmed_authors":["Hildreth V","Ahmad T","Watson H","Jones C","Satsangi J","Young GR","Parkes M","Hart A","Lees R","Kennedy NA","Stewart CJ","Lamb CA","Lees CW","Irving PM","Powell N","Marchesi JR","Doona M","Raine T","Frith K","Liddle T","Speight RA","Wyatt NJ","Tilling N","Lawrence S","Doyle JA","King A","Allerton D","Masi AC","McGregor N","Lindsay JO","Whelan K","Jostins-Dean L","Wood R","Prescott NJ"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of intestinal biopsy tissue preservation methods to facilitate large-scale mucosal microbiota research.","description":"<h4>Background</h4>Large-scale multicentre studies are needed to understand complex relationships between the gut microbiota, health and disease. Interrogating the mucosal microbiota may identify important biology not captured by stool analysis. Gold standard tissue cryopreservation ('flash freezing') limits large-scale study feasibility. We aimed to compare gut microbiota in gold standard and pragmatic mucosal biopsy storage conditions.<h4>Methods</h4>We collected endoscopic recto-sigmoid biopsies from 20 adults with inflammatory bowel disease. Biopsies were preserved using three methods: (i) flash freezing (most proximal and distal biopsy sites); (ii) nucleic acid preservative reagents (QIAGEN Allprotect®, Invitrogen RNAlater™, and Zymo DNA/RNA Shield™); and (iii) formalin fixation with ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-06-02T20:50:59.055Z","creation":"2025-04-05T11:34:40.757Z"},"accession":"S-EPMC11751561","cross_references":{"pubmed":["39742562"],"doi":["10.1016/j.ebiom.2024.105550"]}}