{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nago M"],"funding":["Japan Society for the Promotion of Science"],"pagination":["e12622"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11751902"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(1)"],"pubmed_abstract":["<h4>Purpose</h4>This study aimed to investigate the molecular mechanisms associated with chromosome segregation errors caused by intrinsic oxidative stress during in vitro oocyte maturation (IVM) using oocytes from <i>Sod1</i>-deficient (<i>Sod1</i>KO) mice.<h4>Methods</h4>Ovulated or in vitro matured cumulus-cells oocyte complexes (COCs) were collected from wild-type (WT) and <i>Sod1</i>KO mice and evaluated chromosome alignment, chromosome segregation, meiotic progression, and BUBR1 and REC8 protein expression levels.<h4>Results</h4>In 21% O<sub>2</sub> IVM, the <i>Sod1</i>KO had significantly higher frequencies of chromosome misalignment and segregation errors compared to the WT, and they also reached Germinal Vesicle Break Down (GVBD) and M I stages peak earlier and showed a shorter M "],"journal":["Reproductive medicine and biology"],"pubmed_title":["<i>Sod1</i> deficiency in mouse oocytes during in vitro maturation increases chromosome segregation errors with a reduced BUBR1 at kinetochore."],"pmcid":["PMC11751902"],"funding_grant_id":["24580405","21580342"],"pubmed_authors":["Ishii M","Odajima K","Yanai M","Nago M","Sato Y","Kimura N"],"additional_accession":[]},"is_claimable":false,"name":"<i>Sod1</i> deficiency in mouse oocytes during in vitro maturation increases chromosome segregation errors with a reduced BUBR1 at kinetochore.","description":"<h4>Purpose</h4>This study aimed to investigate the molecular mechanisms associated with chromosome segregation errors caused by intrinsic oxidative stress during in vitro oocyte maturation (IVM) using oocytes from <i>Sod1</i>-deficient (<i>Sod1</i>KO) mice.<h4>Methods</h4>Ovulated or in vitro matured cumulus-cells oocyte complexes (COCs) were collected from wild-type (WT) and <i>Sod1</i>KO mice and evaluated chromosome alignment, chromosome segregation, meiotic progression, and BUBR1 and REC8 protein expression levels.<h4>Results</h4>In 21% O<sub>2</sub> IVM, the <i>Sod1</i>KO had significantly higher frequencies of chromosome misalignment and segregation errors compared to the WT, and they also reached Germinal Vesicle Break Down (GVBD) and M I stages peak earlier and showed a shorter M ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan-Dec","modification":"2026-06-12T05:17:44.049Z","creation":"2025-04-05T00:25:08.222Z"},"accession":"S-EPMC11751902","cross_references":{"pubmed":["39845481"],"doi":["10.1002/rmb2.12622"]}}