biostudies-literatureMi Sthe National Natural Science Foundation of China10https://www.ebi.ac.uk/biostudies/studies/S-EPMC11754336biostudies-literatureUnknown42(2)<h4>Background</h4>In recent years, the emphasis has shifted to understanding the role of N1-methyladenosine (m1A) in tumor progression as little is known about its regulatory effect on mRNA and its role in the metastasis of colorectal cancer (CRC).<h4>Methods</h4>We performed methylated RNA immunoprecipitation sequencing of tumor tissues and tumor-adjacent normal tissues from three patients with CRC to determine the m1A profile of mRNA in CRC. The expression of diaphanous-related formin 3 (DIAPH3) and its correlation with clinicopathological characteristics of CRC were evaluated using immunohistochemistry and online datasets. The role of DIAPH3 in the migration and invasion of CRC cells was evaluated using wound healing assay, Transwell assay and xenograft metastatic model. The downstreamClinical & experimental metastasism1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19.PMC1175433682073229Hu JChen JChen WMi SXue MXu Zfalsem1A-regulated DIAPH3 promotes the invasiveness of colorectal cancer via stabilization of KRT19.<h4>Background</h4>In recent years, the emphasis has shifted to understanding the role of N1-methyladenosine (m1A) in tumor progression as little is known about its regulatory effect on mRNA and its role in the metastasis of colorectal cancer (CRC).<h4>Methods</h4>We performed methylated RNA immunoprecipitation sequencing of tumor tissues and tumor-adjacent normal tissues from three patients with CRC to determine the m1A profile of mRNA in CRC. The expression of diaphanous-related formin 3 (DIAPH3) and its correlation with clinicopathological characteristics of CRC were evaluated using immunohistochemistry and online datasets. The role of DIAPH3 in the migration and invasion of CRC cells was evaluated using wound healing assay, Transwell assay and xenograft metastatic model. The downstream2025-01-01T00:00:00Z2025 Jan2026-06-02T07:10:01.585Z2025-04-05T12:37:02.873ZS-EPMC117543363984373010.1007/s10585-024-10323-0