biostudies-literatureUnknown15(1)Bege MUniversity of DebrecenHydrogen sulfide (H<sub>2</sub>S) is an endogenous gasotransmitter with cardioprotective and antiviral effects. In this work, new cysteine-selective nucleoside-H<sub>2</sub>S-donor hybrid molecules were prepared by conjugating nucleoside biomolecules with a thiol-activatable dithioacetyl group. 5'-Dithioacetate derivatives were synthesized from the canonical nucleosides (uridine, adenosine, cytidine, guanosine and thymidine), and the putative 5'-thio metabolites were also produced from uridine and adenosine. According to our measurements made with an H<sub>2</sub>S-specific sensor, nucleoside dithioacetates are moderately fast H<sub>2</sub>S donors, the guanosine derivative showed the fastest kinetics and the adenosine derivative the slowest. The antioxidant activity of 5'-thionucleosides Scientific reports2876https://www.ebi.ac.uk/biostudies/studies/S-EPMC11754443biostudies-literatureSynthesis, H<sub>2</sub>S releasing properties, antiviral and antioxidant activities and acute cardiac effects of nucleoside 5'-dithioacetates.PMC11754443Lovas MPriksz DKajtar RWeber JEskeif SBorbas AHerczegh PBege MLekli IBernat BHodek JBereczki INovak LKattoub RGfalseSynthesis, H<sub>2</sub>S releasing properties, antiviral and antioxidant activities and acute cardiac effects of nucleoside 5'-dithioacetates.Hydrogen sulfide (H<sub>2</sub>S) is an endogenous gasotransmitter with cardioprotective and antiviral effects. In this work, new cysteine-selective nucleoside-H<sub>2</sub>S-donor hybrid molecules were prepared by conjugating nucleoside biomolecules with a thiol-activatable dithioacetyl group. 5'-Dithioacetate derivatives were synthesized from the canonical nucleosides (uridine, adenosine, cytidine, guanosine and thymidine), and the putative 5'-thio metabolites were also produced from uridine and adenosine. According to our measurements made with an H<sub>2</sub>S-specific sensor, nucleoside dithioacetates are moderately fast H<sub>2</sub>S donors, the guanosine derivative showed the fastest kinetics and the adenosine derivative the slowest. The antioxidant activity of 5'-thionucleosides 2025-01-01T00:00:00Z2025 Jan2025-04-19T18:22:52.985Z2025-04-19T18:22:52.985ZS-EPMC117544433984390210.1038/s41598-025-85351-1