{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lv L"],"funding":["National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["gkae1236"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11754652"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(2)"],"pubmed_abstract":["Programmable control of bacterial gene expression holds great significance for both applied and academic research. This is particularly true for Streptomyces, a genus of Gram-positive bacteria and major producers of prodigious natural products. Despite that a few inducible regulatory systems have been developed for use in Streptomyces, there is an increasing pursuit to augment the toolkit of high-performance induction systems. We herein report a robust and reversible thermo-inducible bio-switch, designated as StrepT-switch. This bio-switch enables tunable and reversible control of gene expression using physiological temperatures as stimulation inputs. It has been proven successful in highly efficient CRISPR/Cas9-mediated genome engineering, as well as programmable control of antibiotic production and morphological differentiation. The versatility of the device is also demonstrated by thermal induction of a site-specific relaxase ZouA for overproduction of actinorhodin, a blue pigmented polyketide antibiotic. This study showcases the exploration a temperature-sensing module and exemplifies its versatility for programmable control of various target genes in Streptomyces species."],"journal":["Nucleic acids research"],"pubmed_title":["A tunable and reversible thermo-inducible bio-switch for streptomycetes."],"pmcid":["PMC11754652"],"funding_grant_id":["2023YFD1700700","32370077"],"pubmed_authors":["Wang M","Lv L","Zhang Y","Lu Y","Fu Y","Liu S","Wang Y","Niu G","Sun J"],"additional_accession":[]},"is_claimable":false,"name":"A tunable and reversible thermo-inducible bio-switch for streptomycetes.","description":"Programmable control of bacterial gene expression holds great significance for both applied and academic research. This is particularly true for Streptomyces, a genus of Gram-positive bacteria and major producers of prodigious natural products. Despite that a few inducible regulatory systems have been developed for use in Streptomyces, there is an increasing pursuit to augment the toolkit of high-performance induction systems. We herein report a robust and reversible thermo-inducible bio-switch, designated as StrepT-switch. This bio-switch enables tunable and reversible control of gene expression using physiological temperatures as stimulation inputs. It has been proven successful in highly efficient CRISPR/Cas9-mediated genome engineering, as well as programmable control of antibiotic production and morphological differentiation. The versatility of the device is also demonstrated by thermal induction of a site-specific relaxase ZouA for overproduction of actinorhodin, a blue pigmented polyketide antibiotic. This study showcases the exploration a temperature-sensing module and exemplifies its versatility for programmable control of various target genes in Streptomyces species.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-26T04:02:38.082Z","creation":"2025-04-06T10:59:21.659Z"},"accession":"S-EPMC11754652","cross_references":{"pubmed":["39704119"],"doi":["10.1093/nar/gkae1236"]}}