{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["53(2)"],"submitter":["De Simone M"],"funding":["Genentech"],"pubmed_abstract":["This study examined nine prominent commercially available single-cell RNA sequencing (scRNA-seq) kits across four technology groups. Each kit was characterized using peripheral blood mononuclear cells (PBMCs) from a single donor, which enabled consistent assessment of factors such as analytical performance, protocol duration and cost. The Chromium Fixed RNA Profiling kit from 10× Genomics, with its probe-based RNA detection method, demonstrated the best overall performance. The Rhapsody WTA kit from Becton Dickinson exhibited a balance between performance and cost. Importantly, we introduce the read utilization metric, which differentiates scRNA-seq kits based on the efficiency of converting sequencing reads into usable counts. Thus, read utilization is an important feature that substantia"],"journal":["Nucleic acids research"],"pagination":["gkae1186"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11754665"],"repository":["biostudies-literature"],"pubmed_title":["A comprehensive analysis framework for evaluating commercial single-cell RNA sequencing technologies."],"pmcid":["PMC11754665"],"pubmed_authors":["Darmanis S","Au-Yeung A","Shi M","Hoover J","Menon H","Vaidya S","Liang Y","Wu B","Xavier-Magalhaes A","Bennett HM","Chen C","Lear S","Lau J","Kurdoglu A","O'Gorman WE","Lund JM","Le D","De Simone M","Modrusan Z"],"additional_accession":[]},"is_claimable":false,"name":"A comprehensive analysis framework for evaluating commercial single-cell RNA sequencing technologies.","description":"This study examined nine prominent commercially available single-cell RNA sequencing (scRNA-seq) kits across four technology groups. Each kit was characterized using peripheral blood mononuclear cells (PBMCs) from a single donor, which enabled consistent assessment of factors such as analytical performance, protocol duration and cost. The Chromium Fixed RNA Profiling kit from 10× Genomics, with its probe-based RNA detection method, demonstrated the best overall performance. The Rhapsody WTA kit from Becton Dickinson exhibited a balance between performance and cost. Importantly, we introduce the read utilization metric, which differentiates scRNA-seq kits based on the efficiency of converting sequencing reads into usable counts. Thus, read utilization is an important feature that substantia","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-05T10:18:47.04Z","creation":"2025-04-05T10:18:47.04Z"},"accession":"S-EPMC11754665","cross_references":{"pubmed":["39675380"],"doi":["10.1093/nar/gkae1186"]}}