{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rush JS"],"funding":["DOE | SC | Chemical Sciences, Geosciences, and Biosciences Division (Chemical Sciences, Geosciences, and Energy Biosciences)","The Knut and Alice Wallenberg Foundation","BLRD VA","NIDCR NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","Vetenskapsrådet","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NIAID NIH HHS","DOE | SC | Chemical Sciences, Geosciences, and Biosciences Division","U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases","Vetenskapsrådet (Swedish Research Council)","NIGMS NIH HHS"],"pagination":["954"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11754754"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Streptococcus mutans, the causative agent of human dental caries, expresses a cell wall attached Serotype c-specific Carbohydrate (SCC) that is critical for cell viability. SCC consists of a polyrhamnose backbone of →3)α-Rha(1 → 2)α-Rha(1→ repeats with glucose (Glc) side-chains and glycerol phosphate (GroP) decorations. This study reveals that SCC has one predominant and two more minor Glc modifications. The predominant Glc modification, α-Glc, attached to position 2 of 3-rhamnose, is installed by SccN and SccM glycosyltransferases and is the site of the GroP addition. The minor Glc modifications are β-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and α-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor β-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation."],"journal":["Nature communications"],"pubmed_title":["Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide."],"pmcid":["PMC11754754"],"funding_grant_id":["R01 DE028916","2022-03014","DE-SC0015662","R01 AI143690","R24 GM137782","I01 BX005637","IK6 BX006469"],"pubmed_authors":["Korotkova N","Kenner CW","Black I","Deng P","Azadi P","Heiss C","Widmalm G","Rush JS","Zamakhaeva S","Korotkov KV","Murner NR","Morris AJ"],"additional_accession":[]},"is_claimable":false,"name":"Structure and mechanism of biosynthesis of Streptococcus mutans cell wall polysaccharide.","description":"Streptococcus mutans, the causative agent of human dental caries, expresses a cell wall attached Serotype c-specific Carbohydrate (SCC) that is critical for cell viability. SCC consists of a polyrhamnose backbone of →3)α-Rha(1 → 2)α-Rha(1→ repeats with glucose (Glc) side-chains and glycerol phosphate (GroP) decorations. This study reveals that SCC has one predominant and two more minor Glc modifications. The predominant Glc modification, α-Glc, attached to position 2 of 3-rhamnose, is installed by SccN and SccM glycosyltransferases and is the site of the GroP addition. The minor Glc modifications are β-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and α-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor β-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-01T18:05:45.607Z","creation":"2025-04-04T22:07:05.52Z"},"accession":"S-EPMC11754754","cross_references":{"pubmed":["39843487"],"doi":["10.1038/s41467-025-56205-1"]}}