{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li H"],"funding":["the Scientific Research Project of Hunan Provincial Department of Education","the Scientific Research Project of Hunan Provincial Health Commission","Natural Science Foundation of Hunan Province","National Natural Science Foundation of China","Huxiang Youth Talent Support Program","International Centre for Genetic Engineering and Biotechnology"],"pagination":["15"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11756191"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Richter syndrome (RS), characterized by aggressive lymphoma arising from chronic lymphocytic leukaemia (CLL), presents a poor response to treatment and grim prognosis. To elucidate RS mechanisms, paired samples from a patient with DLBCL-RS were subjected to single-cell RNA sequencing (scRNA-seq) and high-throughput chromosome conformation capture (Hi-C) sequencing. Over 10,000 cells were profiled via scRNA-seq, revealing the comprehensive B cell transformation in RS. Hi-C sequencing exposed a unique chromatin architecture in RS, with increased proximal and decreased distal interactions. At the compartment scale, the interaction between B compartments was strengthened in DLBCL cells, while topologically associating domains (TADs) in DLBCL had elevated intra-TAD and reduced inter-TAD contact"],"journal":["Biomarker research"],"pubmed_title":["Aberrant c-AMP signalling in richter syndrome revealed by single-cell transcriptome and 3D chromatin analysis."],"pmcid":["PMC11756191"],"funding_grant_id":["32270791","2024JJ6566","CRP/CHN22-03_EC","2022RC1205","C202303046332","2024JJ6549","2023JJ60427","23A0018","82070175"],"pubmed_authors":["Peng H","Li H","Sheng Y","Li J","Wang P","Luo M","Xing C","Zhan Y"],"additional_accession":[]},"is_claimable":false,"name":"Aberrant c-AMP signalling in richter syndrome revealed by single-cell transcriptome and 3D chromatin analysis.","description":"Richter syndrome (RS), characterized by aggressive lymphoma arising from chronic lymphocytic leukaemia (CLL), presents a poor response to treatment and grim prognosis. To elucidate RS mechanisms, paired samples from a patient with DLBCL-RS were subjected to single-cell RNA sequencing (scRNA-seq) and high-throughput chromosome conformation capture (Hi-C) sequencing. Over 10,000 cells were profiled via scRNA-seq, revealing the comprehensive B cell transformation in RS. Hi-C sequencing exposed a unique chromatin architecture in RS, with increased proximal and decreased distal interactions. At the compartment scale, the interaction between B compartments was strengthened in DLBCL cells, while topologically associating domains (TADs) in DLBCL had elevated intra-TAD and reduced inter-TAD contact","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-04T02:09:31.706Z","creation":"2025-04-04T02:09:31.706Z"},"accession":"S-EPMC11756191","cross_references":{"pubmed":["39849544"],"doi":["10.1186/s40364-024-00723-5"]}}