{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han S"],"funding":["?333 Project? of Jiangsu Province","National Center for Research Resources","Wisconsin Alumni Research Foundation","NIA NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NCRR NIH HHS","NIH Office of the Director","University of Wisconsin-Madison","National Natural Science Foundation of China","National Institute of General Medical Sciences","National Institute on Aging","School of Pharmacy, University of Wisconsin?Madison","Division of Chemistry","NIDDK NIH HHS","NIGMS NIH HHS","Qinglan Project of Jiangsu Province of China","NIH HHS"],"pagination":["3870-3878"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11757003"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["96(9)"],"pubmed_abstract":["Gut microbiota can regulate host brain functions and influence various physiological and pathological processes through the brain-gut axis. To systematically elucidate the intervention of different gut environments on different brain regions, we implemented an integrated approach that combines 11-plex DiLeu isobaric tags with a \"BRIDGE\" normalization strategy to comparatively analyze the proteome of six brain regions in germ-free (GF)- and conventionally raised (ConvR)-mice. A total of 5945 proteins were identified and 5656 were quantifiable, while 1906 of them were significantly changed between GF- and ConvR-mice; 281 proteins were filtered with FC greater than 1.2 in at least one brain region, of which heatmap analysis showed clear protein profile disparities, both between brain regions "],"journal":["Analytical chemistry"],"pubmed_title":["11-Plex DiLeu Isobaric Labeling Enables Quantitative Assessment of Brain Region Protein Association Networks Impacted by the Gut Microbiome."],"pmcid":["PMC11757003"],"funding_grant_id":["R01DK071801","R01 AG078794","S10RR029531","CHE-2108223","S10 OD025084","S10 OD028473","81973450","R21 AG065728","R01 DK071801","R01 AG052324","S10OD025084","S10OD028473","R01AG052324","R01 DK108259","R21AG065728","P41 GM108538","DK108259","S10 RR029531","P41GM108538"],"pubmed_authors":["Li Z","Huang J","Cui Y","Frost DC","Liu R","Han S","Rey FE","Li L","Shi Y"],"additional_accession":[]},"is_claimable":false,"name":"11-Plex DiLeu Isobaric Labeling Enables Quantitative Assessment of Brain Region Protein Association Networks Impacted by the Gut Microbiome.","description":"Gut microbiota can regulate host brain functions and influence various physiological and pathological processes through the brain-gut axis. To systematically elucidate the intervention of different gut environments on different brain regions, we implemented an integrated approach that combines 11-plex DiLeu isobaric tags with a \"BRIDGE\" normalization strategy to comparatively analyze the proteome of six brain regions in germ-free (GF)- and conventionally raised (ConvR)-mice. A total of 5945 proteins were identified and 5656 were quantifiable, while 1906 of them were significantly changed between GF- and ConvR-mice; 281 proteins were filtered with FC greater than 1.2 in at least one brain region, of which heatmap analysis showed clear protein profile disparities, both between brain regions ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Mar","modification":"2026-06-02T13:14:44.395Z","creation":"2025-04-04T02:09:20.99Z"},"accession":"S-EPMC11757003","cross_references":{"pubmed":["38373348"],"doi":["10.1021/acs.analchem.3c05327"]}}