biostudies-literatureSardarabadi PNational Science and Technology CouncilInterdisciplinary Integration of Advanced Medical Technology (Medical+X) Program4354-4367https://www.ebi.ac.uk/biostudies/studies/S-EPMC11758792biostudies-literatureUnknown17(3)Interleukin 6 (IL-6), produced by immune cells, is crucial in promoting T cell trafficking to infection and inflammation sites, influencing various physiological and pathological processes. Concentrations of IL-6 and other cytokines and chemokines can influence T cell differentiation and activation. Understanding the dual faces of IL-6 within the tumor microenvironment is crucial to understanding its role. A flow-based microsystem was designed to investigate CD4⁺ T cell activation in response to different IL-6 gradients in an under-control 3D culture. The study found that cancer cells' response to varying IL-6 concentrations was dynamic and dose-sensitive, with immune cell migration rates showing sensitivity to the IL-6 gradient. A549 cell expansion increases gradually and time-ACS applied materials & interfacesInvestigating T Cell Immune Dynamics and IL-6's Duality in a Microfluidic Lung Tumor Model.PMC11758792NSTC 112-2223-E-007-011NSTC 113-2221-E-007-076113QF003E1Liu CHSardarabadi PLee KYSun WLKojabad AAfalseInvestigating T Cell Immune Dynamics and IL-6's Duality in a Microfluidic Lung Tumor Model.Interleukin 6 (IL-6), produced by immune cells, is crucial in promoting T cell trafficking to infection and inflammation sites, influencing various physiological and pathological processes. Concentrations of IL-6 and other cytokines and chemokines can influence T cell differentiation and activation. Understanding the dual faces of IL-6 within the tumor microenvironment is crucial to understanding its role. A flow-based microsystem was designed to investigate CD4⁺ T cell activation in response to different IL-6 gradients in an under-control 3D culture. The study found that cancer cells' response to varying IL-6 concentrations was dynamic and dose-sensitive, with immune cell migration rates showing sensitivity to the IL-6 gradient. A549 cell expansion increases gradually and time-2025-01-01T00:00:00Z2025 Jan2025-04-05T00:25:29.895Z2025-04-05T00:25:29.895ZS-EPMC117587923947128310.1021/acsami.4c09065