{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Saito MA"],"funding":["NIGMS NIH HHS"],"pubmed_abstract":["<i>Pseudomonas aeruginosa</i> is a major contributor to human infections and is widely distributed in the environment. Its ability for growth under aerobic and anaerobic conditions provides adaptability to environmental changes and to confront immune responses. We applied high-throughput native 2-dimensional metalloproteomics to <i>P. aeruginosa</i> to examine how use of iron within the metallome responds to oxic and anoxic conditions. Metalloproteomic analyses revealed four major iron peaks, each comprised of metalloproteins with synergistic functions, including: 1) respiratory and metabolic enzymes, 2) oxidative stress response enzymes, 3) DNA synthesis and nitrogen assimilation enzymes, and 4) denitrification enzymes and related copper enzymes. Three ferritins co-eluted with the first a"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.01.15.633287"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11760780"],"repository":["biostudies-literature"],"pubmed_title":["Detection of Iron Protein Supercomplexes in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; by Native Metalloproteomics."],"pmcid":["PMC11760780"],"funding_grant_id":["R01 GM135709"],"pubmed_authors":["McIlvin MR","Saito MA"],"additional_accession":[]},"is_claimable":false,"name":"Detection of Iron Protein Supercomplexes in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; by Native Metalloproteomics.","description":"<i>Pseudomonas aeruginosa</i> is a major contributor to human infections and is widely distributed in the environment. Its ability for growth under aerobic and anaerobic conditions provides adaptability to environmental changes and to confront immune responses. We applied high-throughput native 2-dimensional metalloproteomics to <i>P. aeruginosa</i> to examine how use of iron within the metallome responds to oxic and anoxic conditions. Metalloproteomic analyses revealed four major iron peaks, each comprised of metalloproteins with synergistic functions, including: 1) respiratory and metabolic enzymes, 2) oxidative stress response enzymes, 3) DNA synthesis and nitrogen assimilation enzymes, and 4) denitrification enzymes and related copper enzymes. Three ferritins co-eluted with the first a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-04-04T01:40:12.92Z","creation":"2025-04-04T01:40:12.92Z"},"accession":"S-EPMC11760780","cross_references":{"pubmed":["39868235"],"doi":["10.1101/2025.01.15.633287"]}}