{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang R"],"funding":["Guangzhou Key Laboratory of Biological Targeting Diagnosis and Therapy","Key Laboratory of Guangdong Higher Education Institutes","National Natural Science Foundation of China","Science and Technology Development Fund","Science and Technology Projects in Guangzhou"],"pagination":["gkaf001"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11760979"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(3)"],"pubmed_abstract":["Cell fate determination at the chromatin level is not fully comprehended. Here, we report that c-JUN acts on chromatin loci to limit mesoderm cell fate specification as cells exit pluripotency. Although c-JUN is widely expressed across various cell types in early embryogenesis, it is not essential for maintaining pluripotency. Instead, it functions as a repressor to constrain mesoderm development while having a negligible impact on ectoderm differentiation. c-JUN interacts with MBD3-NuRD complex, which helps maintain chromatin in a low accessibility state at mesoderm-related genes during the differentiation of human pluripotent stem cells into mesoderm. Furthermore, c-JUN specifically inhibits the activation of key mesoderm factors, such as EOMES and GATA4. Knocking out c-JUN or inhibiting"],"journal":["Nucleic acids research"],"pubmed_title":["c-JUN: a chromatin repressor that limits mesoderm differentiation in human pluripotent stem cells."],"pmcid":["PMC11760979"],"funding_grant_id":["2023A03J0045","0093/2024/AFJ","2021KSYS009","32270574","202201020379","2023A04J0156","92068201"],"pubmed_authors":["Li G","Chen J","Li D","Xiang D","Zhang X","Hutchins AP","Qin D","Zhang R","Wang Z","Su H","Zhang Q","Pei D"],"additional_accession":[]},"is_claimable":false,"name":"c-JUN: a chromatin repressor that limits mesoderm differentiation in human pluripotent stem cells.","description":"Cell fate determination at the chromatin level is not fully comprehended. Here, we report that c-JUN acts on chromatin loci to limit mesoderm cell fate specification as cells exit pluripotency. Although c-JUN is widely expressed across various cell types in early embryogenesis, it is not essential for maintaining pluripotency. Instead, it functions as a repressor to constrain mesoderm development while having a negligible impact on ectoderm differentiation. c-JUN interacts with MBD3-NuRD complex, which helps maintain chromatin in a low accessibility state at mesoderm-related genes during the differentiation of human pluripotent stem cells into mesoderm. Furthermore, c-JUN specifically inhibits the activation of key mesoderm factors, such as EOMES and GATA4. Knocking out c-JUN or inhibiting","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-02T20:37:25.517Z","creation":"2025-04-03T23:57:17.033Z"},"accession":"S-EPMC11760979","cross_references":{"pubmed":["39876710"],"doi":["10.1093/nar/gkaf001"]}}