biostudies-literatureHe Hthe Program for Waterfowl Industry Technology System Innovation Team of Sichuan Provincethe International Cooperation Project of Sichuan Provincethe National Key R&D Program of Chinathe Innovation and Demonstration of Industry and Education Integration in Feed Industrial Chain Transformation and Upgradation of Sichuan Provincethe National Key R&D Program of Chinathe National Natural Science Foundation of China284https://www.ebi.ac.uk/biostudies/studies/S-EPMC11761271biostudies-literatureUnknown15(2)<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint anaAnimals : an open access journal from MDPITranscriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells.PMC11761271322026762023YFD1300304SCCXTD-2024-252022YFH00352212129406Liu HHu SHe HLin YLi LWang JLiao LXie HXia LHu JZhang XHan CYuan XWang ZfalseTranscriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells.<i>PLIN1</i>, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of <i>PLIN1</i> in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched (<i>p</i> < 0.05) in pathways related to biological processes (BPs), particularly those associated with the regulation of cellular lipid metabolism and oxidative stress. KEGG analysis further identified significant enrichment (<i>p</i> < 0.05) in pathways related to cell apoptosis and the cell cycle. A joint ana2025-01-01T00:00:00Z2025 Jan2026-04-15T03:19:24.454Z2025-04-05T19:20:04.468ZS-EPMC117612713985828410.3390/ani15020284