biostudies-literatureRuan CHwaMei Research Foundation of Ningbo No. 2 HospitalNatural Science Foundation of Ningbo MunicipalityNatural Science Foundation of Zhejiang Provincethe Major Special Science and Technology Project of Ningbo Citye70597https://www.ebi.ac.uk/biostudies/studies/S-EPMC11761428biostudies-literatureUnknown14(3)<h4>Background</h4>Hepatocellular carcinoma (HCC) is one of the most common and highly lethal cancers worldwide. RIO kinase 1 (RIOK1), a protein kinase/ATPase that plays a key role in regulating translation and ribosome assembly, is associated with a variety of malignant tumors. However, the role of RIOK1 in HCC remains largely unknown.<h4>Methods</h4>Changes in RIOK1 expression in HCC and patient prognosis were evaluated using HCC tissues and public databases. The functional role of RIOK1 in HCC was analyzed by RTCA assay, clonogenic assay, and flow cytometry in vitro, and by mouse tumor xenograft model in vivo. Potential mechanism studies were performed using multi-omics analysis, public database screening, and qRT-PCR assay.<h4>Results</h4>In this study, we found that RIOK1 was elevatedCancer medicineRIOK1: A Novel Oncogenic Driver in Hepatocellular Carcinoma.PMC11761428LY19H1600102023HMKY442021J3142022HMKY592022Z128Yang YRuan CZhang SShen YRu WShang TfalseRIOK1: A Novel Oncogenic Driver in Hepatocellular Carcinoma.<h4>Background</h4>Hepatocellular carcinoma (HCC) is one of the most common and highly lethal cancers worldwide. RIO kinase 1 (RIOK1), a protein kinase/ATPase that plays a key role in regulating translation and ribosome assembly, is associated with a variety of malignant tumors. However, the role of RIOK1 in HCC remains largely unknown.<h4>Methods</h4>Changes in RIOK1 expression in HCC and patient prognosis were evaluated using HCC tissues and public databases. The functional role of RIOK1 in HCC was analyzed by RTCA assay, clonogenic assay, and flow cytometry in vitro, and by mouse tumor xenograft model in vivo. Potential mechanism studies were performed using multi-omics analysis, public database screening, and qRT-PCR assay.<h4>Results</h4>In this study, we found that RIOK1 was elevated2025-01-01T00:00:00Z2025 Feb2026-06-02T12:07:05.678Z2025-04-04T13:12:14.602ZS-EPMC117614283986540610.1002/cam4.70597